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We narrowed to 4,664 results for: crispr c plasmids

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Showing: 4481 - 4500 of 4664 results

  2. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

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  3. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  4. pLEX307_CR-PDHA2S293A

    Plasmid
    #115204
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115204-plasmid-map-sequence-id-231794

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  5. pLEX307_CR-PDHA2

    Plasmid
    #115199
    Purpose
    Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115199-plasmid-map-sequence-id-232425

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  6. pLEX307_CR-PDHA2S291A

    Plasmid
    #115203
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115203-plasmid-map-sequence-id-232429

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  7. LLP979

    Plasmid
    #239901
    Purpose
    CaMV 35S-SynPro-14 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-14::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    239901-plasmid-map-sequence-id-490465

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  14. LLP967

    Plasmid
    #239889
    Purpose
    CaMV 35S-SynPro-02 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-02::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239889-plasmid-map-sequence-id-490756

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  15. LLP965

    Plasmid
    #239887
    Purpose
    Wild type CaMV 35S promoter engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239887-plasmid-map-sequence-id-490754

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  16. LLP966

    Plasmid
    #239888
    Purpose
    CaMV 35S-SynPro-01 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-01::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239888-plasmid-map-sequence-id-490755

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  17. LLP968

    Plasmid
    #239890
    Purpose
    CaMV 35S-SynPro-03 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-03::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239890-plasmid-map-sequence-id-490753

    Enlarge

  18. LLP969

    Plasmid
    #239891
    Purpose
    CaMV 35S-SynPro-04 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-04::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239891-plasmid-map-sequence-id-490752

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  19. LLP970

    Plasmid
    #239892
    Purpose
    CaMV 35S-SynPro-05 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-05::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239892-plasmid-map-sequence-id-490751

    Enlarge

  20. LLP977

    Plasmid
    #239899
    Purpose
    CaMV 35S-SynPro-12 engineered promoter driving the expression of Renilla luciferase
    Depositor
    Ryan Lister
    Article
    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.
    Insert
    CaMV 35S-SynPro-12::Rluc
    Use
    Synthetic Biology
    Tags
    PEST on C-terminus of Rluc
    Mutation
    N/A
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239899-plasmid-map-sequence-id-490750

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Showing: 4481 - 4500 of 4664 results