We narrowed to 59,942 results for: %s

1. pET28a-MH6-Bsu

   Plasmid

   #163911

   Purpose

   Expresses Bsu large fragment for affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   Bsu polymerase
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. scAAV-Cre

   Plasmid

   #177933

   Purpose

   Expresses Cre-recombinase and can be packaged into AAV particles.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Insert

   CAG-Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-uvsX-H6

   Plasmid

   #163913

   Purpose

   Expresses uvsX for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsX
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. scAAV-sgRNA-GFP

   Plasmid

   #177935

   Purpose

   Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCG_IFITM3

   Plasmid

   #179957

   Purpose

   Expression vector for human IFITM3.

   Depositor

   Konstantin Sparrer, Frank Kirchhoff

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM3 (IFITM3 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG_IFITM1

   Plasmid

   #179955

   Purpose

   Expression vector for human IFITM1.

   Depositor

   Konstantin Sparrer, Frank Kirchhoff

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM1 (IFITM1 Human)
   

   Expression

   Mammalian

   Mutation

   P13A

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCG_IFITM2

   Plasmid

   #179956

   Purpose

   Expression vector for human IFITM2.

   Depositor

   Konstantin Sparrer, Frank Kirchhoff

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM2 (IFITM2 Human)
   

   Expression

   Mammalian

   Mutation

   A33V, T41M, V121I

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CS-TRE-BMI1-Ubc-rtTA

   Plasmid

   #198191

   Purpose

   Generation of Expandable megakaryocyte cell lines from human iPS cells

   Depositor

   Hiromitsu Nakauchi

   Article

   Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

   Insert

   hBMI1 (BMI1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1-MCU-Flag-TetOFF

   Plasmid

   #185657

   Purpose

   Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   pTight TRE

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR-BLINK2

    Plasmid

    #117075

    Purpose

    BLINK2 gene in pDONR vector for Gateway cloning system

    Depositor

    Anna Moroni

    Article

    Alberio et al Nat Methods. 2018 Nov;15(11):969-976. do

    Insert

    BLINK2
    

    Use

    Gateway cloning system

    Mutation

    glutamine 112 changed into aspartate (original LO…

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2 puro - CRBN (#1)

    Plasmid

    #166240

    Purpose

    sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52379

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4
    

    Expression

    Mammalian

    Promoter

    OCT4

    Available Since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2 puro - CRBN (#2)

    Plasmid

    #166241

    Purpose

    sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Expression

    Mammalian

    Promoter

    Oct4

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid

    #153062

    Purpose

    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Mutagenesis to insert a stop codon at aa 298 in B…

    Promoter

    EF1α

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. FOR human OCT4 TALEN

    Plasmid

    #52412

    Purpose

    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    HD NG NN NN NN HD NG HD NG HD HD HD NI NG
    

    Use

    TALEN

    Promoter

    CAGGS

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGL3-Promoter-hPE(+)-Luc

    Plasmid

    #172003

    Purpose

    Luciferase Reporter for Human PTEN Enhancer PE - Forward Orientation

    Depositor

    Daniel Herranz

    Article

    Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

    Insert

    Human PE sequence (hPE)
    

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    SV40 Promoter

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-MCU-mCerulean

    Plasmid

    #185809

    Purpose

    Human mitochondrial calcium uniporter with C-terminus mCerulean tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    Mitochondrial calcium uniporter (MCU Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti HsATP10B WT

    Plasmid

    #171770

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT

    Depositor

    Veerle Baekelandt

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Lentiviral

    Mutation

    D433N

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti HsATP10B D433N

    Plasmid

    #171823

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N

    Depositor

    Veerle Baekelandt

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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