We narrowed to 30,307 results for: ple
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Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsR, PhrpLE, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123A)-EYFP
Plasmid#35505PurposeAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorHas ServiceAAV9InserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123APromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS2214
Plasmid#158417PurposeAAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.DepositorAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
GPR25-DuET
Plasmid#213277PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e
Plasmid#90010PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3eDepositorInsertsCBirA*-Cdc25C WT
NBirA*-14-3-3e
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC33
Plasmid#62330PurposesgRNA + 2x MS2 with MCP-VP64 effector for mammalian cellsDepositorInsertssgRNA + 2x MS2 binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2284
Plasmid#158419PurposeAAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.DepositorAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR55
Plasmid#106026PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)DepositorInsertOpto-GPR55 (GPR55 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-SnFR-gamma2-minWPRE
Plasmid#165497PurposeAAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisationDepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
UseAAVPromoterhuman SynapsinAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(CmYLCV)
Plasmid#202014PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterCmYLCVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_1-MS2-Puro
Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 A242V
Plasmid#11022DepositorAvailable SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
FpG5.BFP.Reporter
Plasmid#134967PurposeLentiviral vector for enhancer reporter assayDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromotermini Fgf4 promoterAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC48
Plasmid#62336PurposesgRNA + 1x COM with COM-VP64 effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p
Plasmid#82438PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3pDepositorInserthsa-miR-27a-3p (MIR27A Human)
MutationThree copies hsa-miR-27a-3p Complementary region …Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC620
Plasmid#62282PurposeExpresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cellsDepositorInsertsMCP-VP64
PCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been inactivated by mutatio…PromoterpAdh and pTdh3Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only