We narrowed to 8,514 results for: sgrna

1. pYDE007

   Plasmid

   #194152

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-TRE3G-NLS-tdMCP-mCherry

   Plasmid

   #99909

   Purpose

   For the expression of NLS-tdMCP-mCherry

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdMCP-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEditA

   Plasmid

   #207531

   Purpose

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-TRE3G-NLS-tdPCP-EGFP

   Plasmid

   #99910

   Purpose

   For the expression of NLS-tPMCP-EGFP

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdPCP-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV1326

   Plasmid

   #111435

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p944-SWITCH-OVER insert: EF1a-Puro

   Plasmid

   #217891

   Purpose

   insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CMV-NLS-dCas9-NLS

   Plasmid

   #99908

   Purpose

   For the expression of NLS-dCas9-NLS

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-dCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLib6.4-Aalb_743

   Plasmid

   #176662

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJZC603

    Plasmid

    #62317

    Purpose

    sgRNA with 2x PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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