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pSR40.18

Plasmid

#125084

Purpose

Expression of ompR(1-150aa)-ccaR(140-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)150
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.14

Plasmid

#125081

Purpose

Expression of ompR(1-122aa)-ccaR(119-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)122
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.13

Plasmid

#125080

Purpose

Expression of ompR(1-118aa)-ccaR(115-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)118
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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N229A Lo-MetQ

Plasmid

#118268

Purpose

N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Insert

MetQ (N229A) (metQ E. coli)

Tags

10x His Tag and Enterokinase Cleavage Site

Expression

Bacterial

Mutation

Changed Asparagine (Asn) 229 to Alanine (Ala)

Promoter

T7 Promoter

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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gh182

Plasmid

#106843

Purpose

expression of gRNA targeting GLT6D1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GLT6D1 (GLT6D1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBG1

Plasmid

#111696

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBG1 (TUBG1 Human)

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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gamma-tubulin (334-451)

Plasmid

#104436

Purpose

expressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin fragment 334 to 451 (TUBG1 Human)

Tags

None

Expression

Mammalian

Mutation

deleted a.a. 1 to 333

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSN28

Plasmid

#100113

Purpose

expresses a chimeric cMYC-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSN27

Plasmid

#100112

Purpose

expresses a chimeric cMYC-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to a 'medium' VP16 domain (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSN26

Plasmid

#100111

Purpose

expresses a chimeric cMYC-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to a 'strong' VP16 domain (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG245-Ras-related

Plasmid

#99079

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ras-related (also known as h.18.4a) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edusp5E1

Plasmid

#90122

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of dusp5

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5E1 (dusp5 Zebrafish)

Expression

Bacterial

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dusp5P1Egfp

Plasmid

#90161

Purpose

contains dusp5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdusp5P1gfp

Plasmid

#90126

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dusp5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-RIPK3 V460P

Plasmid

#78826

Purpose

To detect localization of RIPK3 V460P protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

V460P

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-N2-RIPK3 2KR(89,501)

Plasmid

#78824

Purpose

To detect localization of RIPK3 2KR(89,501) protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

K89R, K501R

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-N2-RIPK3 2KR(55,363)

Plasmid

#78823

Purpose

To detect localization of RIPK3 2KR(55,363) protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

K55R, K363R

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3 2KR(89,501)

Plasmid

#78820

Purpose

To overexpress RIPK3 2KR(89,501) in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K89R, K501R

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3 2KR(55,363)

Plasmid

#78819

Purpose

To overexpress RIPK3 2KR(55,363) in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K55R, K363R

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3 S199A

Plasmid

#78818

Purpose

To overexpress RIPK3 S199A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S199A

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only