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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4521 - 4540 of 12282 results

  1. Dronpa-FHA1

    Plasmid
    #87711
    Purpose
    Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dronpa-FHA1
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87711-plasmid-map-sequence-id-176141

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  7. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid
    #208965
    Purpose
    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208965-plasmid-map-sequence-id-415813

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  9. PAGFP_NS2B3

    Plasmid
    #195755
    Purpose
    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
    Depositor
    Partha Mondal
    Publication
    Varghese et al bioRxiv 2022.12.18.520514
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dengue (Type-2) NS2B3
    Tags
    PA-GFP
    Expression
    Mammalian
    Mutation
    No mutations
    Promoter
    CMV
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195755-plasmid-map-sequence-id-384111

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  11. HA-GFP1-10-actin-pcDNA4-TO

    Plasmid
    #118370
    Purpose
    Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.
    Depositor
    Maria Vartiainen
    Publication
    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    actin beta (ACTB Human)
    Tags
    HA-GFP1-10
    Expression
    Mammalian
    Promoter
    CMV/TO
    Available since
    May 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118370-plasmid-map-sequence-id-229456

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  12. pCEP4-Q842gp160

    Plasmid
    #100931
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100931-plasmid-map-sequence-id-202601

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  14. pcDNA3-RAB-AKARev(T/A)

    Plasmid
    #118479
    Purpose
    Negative-control mutant for RAB-AKARev biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-AKARev(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118479-plasmid-map-sequence-id-230370

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  15. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

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  16. pAAV-CAG-FLEx-iSeroSnFR (AAV9)

    Viral prep
    #128486-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-FLEx-iSeroSnFR (#128486). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEx-iSeroSnFR plasmid DNA. CAG driven, Cre-dependent expression of iSeroSnFR. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Publication
    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31
    Promoter
    CAG
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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Showing: 4521 - 4540 of 12282 results