We narrowed to 13,659 results for: crispr cas9

1. psBIG2abc

   Plasmid

   #229963

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psBIG2ab

   Plasmid

   #229962

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psBIG2abcd

   Plasmid

   #229964

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psBIG2abcde

   Plasmid

   #229965

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. psBIG1d

   Plasmid

   #229960

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psBIG1e

   Plasmid

   #229961

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psBIG1c

   Plasmid

   #229959

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

   Plasmid

   #199496

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. M-ST1n-VP64

   Plasmid

   #48675

   Purpose

   Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Mutation

   nuclease-null (D9A D598A H599A N622A)

   Promoter

   CMV

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF730

    Plasmid

    #121668

    Purpose

    EF1a-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF704

    Plasmid

    #121658

    Purpose

    U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCF712

    Plasmid

    #121663

    Purpose

    U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210749

    Purpose

    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    CjSp D8A Cas9
    Bilbo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6>sgRNA(F+E)

    Plasmid

    #59986

    Purpose

    Empty vector for sgRNA cloning (F+E modified backbone)

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    Ciinte.U6

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDonor_sU6

    Plasmid

    #69351

    Purpose

    sgRNA scaffold and synthetic U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    sU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    synthetic U6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonor_mU6

    Plasmid

    #69350

    Purpose

    sgRNA scaffold and mouse U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    mU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    mU6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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