We narrowed to 15,021 results for: Dos

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p3E-ChBP

Plasmid

#108892

Purpose

Multisite gateway vector for 3' tagging with a nanobody against mCherry

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

ChBP

Use

Gateway Cloning

Available since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Publication

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJB153

Plasmid

#92328

Purpose

Express mEos3.2 tagged ZapA in E coli

Depositor

Jie Xiao

Publication

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

ZapA

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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p5sfGFP

Plasmid

#92024

Purpose

sfGFP reporter plasmid with lac operator in its promoter

Depositor

Brian Pfleger

Publication

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sfGFP

Use

Unspecified

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-3

Plasmid

#90327

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

Plasmid

#83456

Purpose

Human PC1-CTp30 expression cassette with a GST-tag (678)

Depositor

Thomas Weimbs

Publication

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (214-513aa)

Plasmid

#78095

Purpose

mammalian expression of GADD34 214-513 aa

Depositor

Shirish Shenolikar

Publication

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 214-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
mCitrine-USP19 1-1290

Plasmid

#78595

Purpose

Express mCitrine-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 1-1290 (USP19 Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
MXS_CMV::NeoR-bGHpA

Plasmid

#62437

Purpose

MXS_chaining vector with CMV::NeoR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with CMV Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82704

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Publication

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L Empty gRNA Expression Cassette

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-FKBP-GFP-HIS3MX6

Plasmid

#183023

Purpose

PCR-based tagging of yeast

Depositor

Santiago Di Pietro

Publication

Lamb et al STAR Protoc. 2022 Apr 14;3(2):101323. do

Insert

FKBP

Use

Yeast genomic targeting

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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hOCT1-miRFP

Plasmid

#188995

Purpose

A mammalian expression plasmid encoding miRFP-tagged human OCT1

Depositor

Jeremy Baskin

Publication

Chiu et al JACS Au. 2022 Nov 28;2(12):2703-2713. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hOCT1 (SLC22A1 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-mEos3.2

Plasmid

#189720

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Publication

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Publication

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDG058

Plasmid

#122840

Purpose

SRDX repressor domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Publication

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SRDX transcriptional repressor domain

Use

Golden gate compatible cloning vector

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMH185

Plasmid

#122839

Purpose

VP64 activator domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Publication

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

VP64 transcriptional activator domain

Use

Golden gate compatible cloning vector

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL2148

Plasmid

#71208

Purpose

pT181 Sense Expression Plasmid

Depositor

Julius Lucks

Publication

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pT181 Sense

Use

Synthetic Biology

Promoter

BBa_J23119

Available since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Publication

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Promoter

T7

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1

Plasmid

#60385

Purpose

Expression of yeast alpha tubulin (TUB1) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only