We narrowed to 15,991 results for: LEA

1. CAG.mCherry-INBox(AAA)-eDHFR

   Plasmid

   #176207

   Purpose

   INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   mCherry-INBoxAAA-eDHFR
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetO::EGFP-hMeikin(8A mut)

   Plasmid

   #174707

   Purpose

   Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH775_pJH_LldR_PlldR-sfGFP.2opal

   Plasmid

   #163734

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding two opal codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.2TGA
   

   Expression

   Bacterial

   Mutation

   gfp with first two leucine codons mutated to amber

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-HA-PGRMC1-1-54

   Plasmid

   #173472

   Purpose

   PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1 (PGRMC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted for amino acids 55-194

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HA-PGRMC1-21-195

   Plasmid

   #173473

   Purpose

   PGRMC1 lacking the N-terminal region upstream of the transmembrane region

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC1 (PGRMC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted for amino acids 1-20

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc120

   Plasmid

   #161950

   Purpose

   Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB4A3-PECF20-B30-mScarlet-I-B15
   

   Expression

   Bacterial

   Promoter

   PECF20

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. IBMc393

   Plasmid

   #161953

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB4A3-T-PT7-B30-mScarlet-I-3T
   

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. IBMc330

   Plasmid

   #161952

   Purpose

   Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSEVA171-PSrpR-B30-mScarlet-I-Ter
   

   Expression

   Bacterial

   Promoter

   PSrpR

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. IBMc050

   Plasmid

   #161938

   Purpose

   Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
   

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. IBMc259

    Plasmid

    #161951

    Purpose

    Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
    

    Expression

    Bacterial

    Promoter

    PTet*

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nup54 no FG-mEGFP494(2)

    Plasmid

    #164271

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nup54 no FG-mEGFP494(1)

    Plasmid

    #164270

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

    Plasmid

    #149346

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbc

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

    Plasmid

    #149347

    Purpose

    Mammalian expression vector for SpyCas9 and puromycin resistance

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-5U6-sgRNAs-hsyn-EGFP

    Plasmid

    #112213

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    sgRNAs
    

    Use

    AAV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

    Plasmid

    #91802

    Purpose

    Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEM_HcBOP1ainsitu

    Plasmid

    #136529

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Houttuynia cordata BLADE-ON-PETIOLE homolog1a
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM_ZmaBOP1binsitu

    Plasmid

    #136531

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Zoysia matrella BLADE-ON-PETIOLE homolog1b
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM_ZmaBOP2binsitu

    Plasmid

    #136532

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Zoysia matrella BLADE-ON-PETIOLE homolog2b
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM_HcBOP1binsitu

    Plasmid

    #136530

    Purpose

    Template for generating in situ hybridization probe

    Depositor

    Junko Kyozuka

    Article

    Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

    Insert

    Houttuynia cordata BLADE-ON-PETIOLE homolog1b
    

    Use

    Cloning vector

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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