We narrowed to 64,554 results for: cell;

1. pCALNL-mutKir2.1

   Plasmid

   #125579

   Purpose

   Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A non-conducting mutant (GYG -> AAA).

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCCLc-U6-shHMGA2.2-PGK-dTomato

   Plasmid

   #89605

   Purpose

   Silencing Hmga2 in human cells

   Depositor

   Fernando Fierro

   Article

   Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

   Insert

   shRNA targeting HMGA2 (HMGA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cry2 mCherry GRK3 ct in pcDNA3.1

   Plasmid

   #64213

   Purpose

   mCherry fused between Cry2 and GRK3 ct to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   GRK3 ct (GRK3 Human)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW TetO HA-AMPKa1 AS

   Plasmid

   #69825

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

   Plasmid

   #74919

   Purpose

   Mammalian expression of mKlf2 and mNanog-mCherry

   Depositor

   Jose Silva

   Article

   dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

   Inserts

   Klf2 (Klf2 Mouse)
   Nanog (Nanog Mouse)
   

   Tags

   T2A and mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. EBL1-bio

   Plasmid

   #47792

   Purpose

   Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised EBL1
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAFNF-PSDd1.2-GFP

   Plasmid

   #125581

   Purpose

   Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   PSDd1.2-GFP (Dlg4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE2-TetOminiCMV-Klf4

   Plasmid

   #136613

   Purpose

   Dox-inducible lentiviral vector expressing mouse Klf4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-TetO-Gfi1b

    Plasmid

    #131592

    Purpose

    doxycycline-inducible expression of mouse GFI1B in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Gfi1b (Gfi1b Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-CARMA2cl-FLAG

    Plasmid

    #97077

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pf92-bio

    Plasmid

    #47728

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf92
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMP108 Nanog-Venus-2a-mCherry

    Plasmid

    #159743

    Purpose

    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

    Depositor

    Sergi Regot

    Article

    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    Venus-2a-mCherry

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. YF-Cav1s

    Plasmid

    #171462

    Purpose

    Expresses YF-Cav1s, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP-FKBP12-Caveolin1(61-178 aa)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mActZ-FY

    Plasmid

    #171463

    Purpose

    Expresses mActZ-FY, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCGN-HCF-1-N450

    Plasmid

    #92321

    Purpose

    N-term 1-450 of HCF-1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 (HCFC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2-450. Note that a SRRASGTYGRV amino-terminal …

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJD-KLF4-ER

    Plasmid

    #34596

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    Myc epitope tag and estrogen receptor fusion

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Luc-Nanog-3'UTR

    Plasmid

    #63893

    Purpose

    Luciferase reporter of mouse wildtype Nanog 3'UTR

    Depositor

    Lin He

    Article

    Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Expression

    Mammalian

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pECE M2-SH3PXD2A 8 serines mutated to alanines

    Plasmid

    #69816

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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