We narrowed to 8,355 results for: construct

1. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

   Plasmid

   #86451

   Purpose

   Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)

   Depositor

   Gregor Bucher

   Insert

   Hsp_p-GAL4Delta-SV40;attp
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

   Plasmid

   #86455

   Purpose

   Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1203C-CMV-5’+3'DR-EGFP-bGHpolyA

   Plasmid

   #221454

   Purpose

   EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1203R-CMV-3'dmDR-EGFP-bGHpolyA

   Plasmid

   #220962

   Purpose

   EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2/shαSMA/GFP4_Seq2/clone1

   Plasmid

   #201402

   Purpose

   Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Insert

   alpha-SMA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR_Wheat_live_Cas9

   Plasmid

   #126880

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa

   Plasmid

   #86452

   Purpose

   UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFs

   Depositor

   Gregor Bucher

   Insert

   UAS-Tc'hsp_p-tGFP-SV40
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCASCADE-proD

   Plasmid

   #65820

   Purpose

   Const. Promoter ProD targetting RNA E. coli Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   proD guide
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induc…

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mem-mCherry-pTRIP

   Plasmid

   #163521

   Purpose

   Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   membrane targeted mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. nuc-mCherry-pTRIP

    Plasmid

    #163520

    Purpose

    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    nucleus targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. DH-BB(5'SIN;TE12ORF)

    Plasmid

    #72309

    Purpose

    Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus

    Depositor

    Anthony Zador

    Insert

    DH-BB(5'SIN;TE12ORF)
    

    Use

    Sindbis virus helper construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ251-GERC

    Plasmid

    #47441

    Purpose

    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.

    Depositor

    George Church

    Insert

    Gene Expression Reporter Construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. RARS_pNIC-Bio3

    Plasmid

    #153055

    Purpose

    Expression construct for producing Avi tagged Arginyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    RARS (RARS1 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

    Plasmid

    #86453

    Purpose

    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-ROSAf

    Plasmid

    #54323

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-ROSAr

    Plasmid

    #54324

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-Basic-OPN-1200

    Plasmid

    #11996

    Depositor

    Steven Johnson

    Insert

    Osteopontin (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The following construct contains the OPN promoter…

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

    Plasmid

    #86454

    Purpose

    Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. NARS_pNIC-Bio3

    Plasmid

    #153054

    Purpose

    Expression construct for producing Avi tagged Asparaginyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    NARS (NARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid

    #172298

    Purpose

    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

    Depositor

    Patrik Verstreken

    Insert

    CRE-T2A-PuroR
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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