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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMNK56 - piRNAi 21ur-1224 - him-8

Plasmid

#159819

Purpose

piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

Depositor

Christian Froekjaer-Jensen

Publication

Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

Insert

him-8 piRNAi 21ur-1224

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Gal4-entry

Plasmid

#128014

Purpose

Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Publication

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4-DBD-3xFlag

Use

Gal4 entry vector

Tags

Gal4-DBD-3xFlag

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNB769

Plasmid

#60138

Purpose

Red click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Expression

Yeast

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-MAPRE1

Plasmid

#31589

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-DR RecR

Plasmid

#12617

Depositor

SeWon Suh

Publication

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Tags

His

Expression

Bacterial

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-HA_N-human Prkd2

Plasmid

#137850

Purpose

Express Prkd2 in mammalian cells

Depositor

Bruce Beutler

Publication

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Protein kinase d2 (PRKD2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA4 (MSW#517)

Plasmid

#208070

Purpose

Expression of GFP-tagged hsRPA4 in human cells

Depositor

Marc Wold

Publication

Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

Insert

RPA4 (RPA4 Human)

Tags

GFP

Expression

Mammalian

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Publication

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Tags

3x FLAG and mCherry

Expression

Mammalian

Promoter

human Synapsin

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-Zmat3

Plasmid

#167343

Purpose

Expresses HA-tagged Zmat3

Depositor

Laura Attardi

Publication

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

Zinc finger matrin-type 3 (Zmat3 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 PB1mut

Plasmid

#204577

Purpose

Expresses 3xFLAG-p62 PB1mut in mammalian cells

Depositor

Masaaki Komatsu

Publication

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Expression

Mammalian

Mutation

K7A/D69A

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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DDX5 D248N V5

Plasmid

#199577

Purpose

Expresses helicase dead DDX5 cloned into pLenti puro

Depositor

Michael Kastan

Publication

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

DDX5 (DDX5 Human)

Tags

V5

Expression

Mammalian

Mutation

D248N

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Publication

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso2E335A

Plasmid

#159588

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Publication

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MfPylRS (human-opti)

Plasmid

#164081

Purpose

To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Publication

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MfPylRS(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

Plasmid

#118804

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Publication

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 48-232 (#182)

Plasmid

#99920

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 48-232

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Publication

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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