We narrowed to 16,622 results for: puromycin

1. PX459-APP-KO

   Plasmid

   #176487

   Purpose

   To knockout APP gene

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

   Plasmid

   #129632

   Purpose

   Visualization of Akt and ERK activity

   Depositor

   Dorus Gadella

   Article

   Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

   Insert

   PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BRCA2 A11.2 gRNA

   Plasmid

   #90550

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A11.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. betaTrCP mVenus mutant reporter

   Plasmid

   #192459

   Purpose

   Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CdC25B
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338 of CDC25B. betaTrCP degron sequence (aa…

   Promoter

   EF1alpha

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPM-D4 miR30-TRIM28 ts1

   Plasmid

   #115862

   Purpose

   TRIM28 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   TRIM28 shRNA (TRIM28 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-2A-GFP-IRESpuro2

   Plasmid

   #183746

   Purpose

   Expresses H2B-2A-GFP

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   GFP with 2A protease cleavage site between H2B an…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi 41BBL CD86 HLA-A201

   Plasmid

   #183248

   Purpose

   Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Inserts

   CD86, 41BBL
   HLA-A2 (HLA-A Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-IP HA-Parkin

   Plasmid

   #38248

   Depositor

   Noboru Mizushima

   Article

   Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

   Insert

   Parkin (PRKN Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBpuro-ErbB1

   Plasmid

   #197358

   Purpose

   Encoding human ErbB1.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   EGFR (EGFR Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-TetOne-Puro-LbNOX-FLAG

    Plasmid

    #218631

    Purpose

    This plasmid contains LbNOX under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-PIG-Cre

    Plasmid

    #50935

    Purpose

    retroviral expression of Cre recombinase

    Depositor

    Pier Pandolfi

    Article

    Chen et al Nature. 2005 Aug 4;436(7051):725-30.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ shTREX1

    Plasmid

    #127700

    Purpose

    Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shTREX1 (Trex1 Human, Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ-5S-F30-Mango IV

    Plasmid

    #127584

    Purpose

    Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    5S rRNA with F30 folding scaffold and Mango IV tag
    

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV MCL1 Puro

    Plasmid

    #140746

    Purpose

    Expresses MCL1 in mammalian cells

    Depositor

    Scott Dixon

    Article

    Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. sg2.02 16x(MS2) MUC4.1

    Plasmid

    #101154

    Purpose

    gRNA with 16 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX_TRE3G_DD::mCherry

    Plasmid

    #108679

    Purpose

    Tunable system for controlled gene expression and protein stability in mammalian cells

    Depositor

    Lucia Marucci

    Article

    Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

    Insert

    Destabilizing Domain (DD) mCherry fusion protein
    

    Use

    Lentiviral

    Tags

    DD::mCherry fusion protein

    Expression

    Mammalian

    Promoter

    Doxycycline inducible

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPM-D4 miR30-TRIM28 ts3

    Plasmid

    #115864

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMPP-mCherry-mTrim21

    Plasmid

    #104971

    Purpose

    Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (Trim21 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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