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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 4561 - 4580 of 14359 results

  2. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid
    #165450
    Purpose
    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter
    Depositor
    Elvir Becirovic
    Publication
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and short human rhodopsin promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165450-plasmid-map-sequence-id-331048

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  8. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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  11. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid
    #102895
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9VPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102895-plasmid-map-sequence-id-194733

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  14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide4

    Plasmid
    #125459
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125459-plasmid-map-sequence-id-251390

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  15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide3

    Plasmid
    #125458
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125458-plasmid-map-sequence-id-251380

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  16. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide2

    Plasmid
    #125457
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125457-plasmid-map-sequence-id-251379

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  17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide1

    Plasmid
    #125456
    Purpose
    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125456-plasmid-map-sequence-id-251378

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Showing: 4561 - 4580 of 14359 results