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Plasmid#139042PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertmscarlet (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF88
Plasmid#139039PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJF6
Plasmid#139040PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertgfp (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM03
Plasmid#139043PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertdendra2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcC)
Plasmid#176197PurposegfcC protein expressionDepositorInsertgfcC
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcB)
Plasmid#176196PurposegfcB protein expressionDepositorInsertgfcB
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSDH57
Plasmid#139047PurposeMosSCI SapTrap assembly: Cassette 3A for 5-cassette systemDepositorInsertmScarlet (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM12
Plasmid#139035PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertdendra2 (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF130
Plasmid#139041PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertmkate2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF222
Plasmid#139032PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertmkate2 (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSDH51
Plasmid#139045PurposeMosSCI SapTrap assembly: Cassette 3A for 5-cassette systemDepositorInserthalotag (no STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM04
Plasmid#139046PurposeMosSCI SapTrap assembly: Cassette 3A for 5-cassette systemDepositorInsertmkate2 (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM10
Plasmid#139034PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertmScarlet (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM05
Plasmid#139050PurposeMosSCI SapTrap assembly: Cassette 3B for 5-cassette systemDepositorInsertmkate2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
UseTagsHis-SUMOExpressionBacterialMutationC110APromoterAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
UseTagsHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
UseTagsHis-Twin Strep-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-Hygro
Plasmid#190805PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance geneDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationSwedish (KM670/671NL)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pXF276
Plasmid#139048PurposeMosSCI SapTrap assembly: Cassette 3B for 5-cassette systemDepositorInsertlov domain (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSDH52
Plasmid#139049PurposeMosSCI SapTrap assembly: Cassette 3B for 5-cassette systemDepositorInsertepdz (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD363
Plasmid#91829PurposeLoxP-flanked SEC donor for the SapTrap cloning systemDepositorInsertLoxP-SEC-LoxP
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD379
Plasmid#91834PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVL-4
Plasmid#104582PurposeContains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.DepositorInsertHuman CL kappa
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD364
Plasmid#91831PurposeLox511I-flanked SEC donor for the SapTrap cloning systemDepositorInsertLox511I-SEC-Lox511I
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD382
Plasmid#91830PurposeLox2272-flanked SEC donor for the SapTrap cloning systemDepositorInsertLox2272-SEC-Lox2272
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSDH61
Plasmid#139033PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertepdz (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
ERAP1
Plasmid#39174PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertERAP1 (ERAP1 Human)
UseBaculovirus expressionTagsTEV-His10-FlagExpressionMutationPromoterpolyhedrinAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPIce-T2A-mCherry
Plasmid#190806PurposeExpress Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationIcelandic (A673T)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
APP_Halo_C_allele
Plasmid#178137PurposeDonor vector for endogenous tagging of human APP at the C-terminus with halotagInsertHalotag (APP Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT271.359
Plasmid#127845Purposeclone A3A CBEs by SapI Golden GateDepositorInsertA3A
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS236
Plasmid#73684PurposeSapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS253
Plasmid#114271PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxTagsExpressionMutation*please see depositor comment belowPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
UseTags6xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD-10xHis)
Plasmid#176192PurposegfcD protein expressionDepositorInsertgfcD
UseTags10xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only