We narrowed to 19,059 results for: 158

1. p663-UBC-miniTurbo-V5-CDK7_IDG-K

   Plasmid

   #158194

   Purpose

   Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK7 (CDK7 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p663-UBC-miniTurbo-V5-CDK10_IDG-K

   Plasmid

   #158171

   Purpose

   Gateway destination clone of CDK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK10 (CDK10 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K

   Plasmid

   #158161

   Purpose

   Gateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   2xV5-miniTurbo-NES
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-CTSL-G418

   Plasmid

   #158468

   Purpose

   Constitutive lentiviral expression of human CTSL.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   CTSL (CTSL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_FLAG.Ollas.VSVg_NGFR

   Plasmid

   #158324

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG.Ollas.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_V5.AU1.VSVg_NGFR

   Plasmid

   #158241

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5.AU1.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX403-EYFP-ccdB-G418

   Plasmid

   #158550

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-SARS-CoV-2 Orf3a

   Plasmid

   #158372

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 Orf3a (ORF3a )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAC3-ISH

   Plasmid

   #15854

   Depositor

   Peter Mombaerts

   Article

   Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

   Insert

   Adenylate Cyclase 3 (Adcy3 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   contains residues 787-1021

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid

    #158758

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    nullCoChR-GCaMP6f-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-mGold-Keratin-N-17

    Plasmid

    #158001

    Purpose

    Visualization of keratin using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    mGold-Keratin-N-17 (KRT18 Human)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPCX mCER-Prx1

    Plasmid

    #158227

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p33Fn

    Plasmid

    #158155

    Purpose

    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Rice codon optimized

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAS1052

    Plasmid

    #158598

    Purpose

    VioA, VioB

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    VioA
    VioB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPCX mCER-Prx2

    Plasmid

    #158225

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-10xMS2 oligo-library based

    Plasmid

    #158200

    Purpose

    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of MS2 from oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. L3691

    Plasmid

    #1587

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    mec7-836, mec7Tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-mGold-Lysosome-N-20

    Plasmid

    #158006

    Purpose

    Visualization of lysosome in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDOC-K-glmS-lacIZ

    Plasmid

    #158061

    Purpose

    Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    lacIZ operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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