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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,217 results for: Alk

Showing: 441 - 460 of 4217 results

  1. pCIneo-AR-S308A

    Plasmid
    #171229
    Purpose
    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)
    Depositor
    Steven Balk
    Article
    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10
    Insert
    human androgen receptor Ser308Ala mutant (AR Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    AR-Ser308Ala (AR-S308A)
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171229-plasmid-map-sequence-id-336012

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  2. pCIneo-AR-S90A

    Plasmid
    #171225
    Purpose
    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)
    Depositor
    Steven Balk
    Article
    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10
    Insert
    human androgen receptor Ser90Ala mutant (AR Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    AR-Ser90Ala (AR-S90A)
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171225-plasmid-map-sequence-id-335918

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  3. Flag-neo-PP1-Alpha-H248K

    Plasmid
    #171268
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    PPP1CA-His248Lys (H248K)
    Promoter
    CMV
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171268-plasmid-map-sequence-id-336095

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  5. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid
    #154285
    Purpose
    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Gateway entry vector
    Tags
    2x FLAG and mKate2
    Expression
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations (HCF-…
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154285-plasmid-map-sequence-id-304861

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  6. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  7. Flag-M4-AR-W741C

    Plasmid
    #171247
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)
    Depositor
    Steven Balk
    Article
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys (AR-W741C)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171247-plasmid-map-sequence-id-336010

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  8. Flag-M4-AR-S81D

    Plasmid
    #171242
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)
    Depositor
    Steven Balk
    Article
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser81Asp (AR-S81D)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171242-plasmid-map-sequence-id-336024

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  12. pCIneo-AR-S81E

    Plasmid
    #171224
    Purpose
    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)
    Depositor
    Steven Balk
    Article
    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10
    Insert
    human androgen receptor Ser81Glu mutant (AR Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    AR-Ser81Glu (AR-S81E)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171224-plasmid-map-sequence-id-336020

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  13. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

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Showing: 441 - 460 of 4217 results