We narrowed to 9,669 results for: Coli

1. pAB172a

   Plasmid

   #156324

   Purpose

   Expresses heterologous rRNA

   Depositor

   Ahmed Badran

   Article

   Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

   Insert

   rRNA operon, orthogonal-antiRBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT antiRBS engineered to orthogonal antiRBS

   Promoter

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TGT-His

   Plasmid

   #138201

   Purpose

   Expresses E. Coli TGT with a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Insert

   E Coli tRNA Guanine Transglycosylase-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA2219

   Plasmid

   #38152

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   DsbC (dsbC E. coli)
   

   Use

   Tags

   N-terminal leader peptide (cloning artifact)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psgRNAc

   Plasmid

   #114006

   Purpose

   express sgRNA, p15A, CRM

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBI

   Plasmid

   #17816

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pKDsgRNA-FRT

   Plasmid

   #183241

   Purpose

   anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

   Depositor

   Karin Schnetz

   Article

   Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

   Insert

   sgRNA-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P-tet

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PAS528 (TJF059)

   Plasmid

   #65602

   Purpose

   pETDuet-1 Containing FadD-His

   Depositor

   Pamela Silver

   Article

   Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

   Insert

   FadD
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLA230

   Plasmid

   #11724

   Depositor

   Lawrence Loeb

   Article

   Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

   Insert

   b-lactamase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GAA for Glu26 changed to TAA (premature stop codo…

   Promoter

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTETDuet_M42FG121VDHFR_R166QTS

    Plasmid

    #191298

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F/G121V DHFR, R166Q TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTrcECT

    Plasmid

    #75477

    Purpose

    Inducible expression of ectABC in E. coli

    Depositor

    Xixian Xie

    Article

    Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

    Insert

    ectABC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMAM-RecG-ST2TaV-EGFP

    Plasmid

    #31275

    Depositor

    Andrew Pierce

    Article

    Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

    Insert

    RecG-ST2-TaV-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EcYbbN pET15b

    Plasmid

    #60690

    Purpose

    Recombinant protein expression of YbbN

    Depositor

    Mark Wilson

    Article

    Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

    Insert

    YbbN
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMAM-RecG-EGFP

    Plasmid

    #31274

    Depositor

    Andrew Pierce

    Article

    Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

    Insert

    RecG-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MetNI (Lm)

    Plasmid

    #78316

    Purpose

    Methionine ABC Transporter

    Depositor

    Douglas Rees

    Article

    Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

    Inserts

    MetI
    MetN
    

    Use

    Tags

    10x Histidine Tag and 6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD33(A-N)

    Plasmid

    #187084

    Purpose

    Medium expression of Complex I with subunit N-HA tagged

    Depositor

    Steven Vik

    Article

    Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

    Insert

    nuoA-N
    

    Use

    Tags

    HA tag nuoN

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBAD33(EFG)

    Plasmid

    #187087

    Purpose

    Medium expression of subunits EFG

    Depositor

    Steven Vik

    Article

    Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

    Insert

    nuoEFG
    

    Use

    Tags

    HA tag nuoG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTETDuet_WTDHFR_WTTS

    Plasmid

    #191282

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW209

    Plasmid

    #113188

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSPK1

    Plasmid

    #18876

    Depositor

    Jay Keasling

    Article

    Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

    Insert

    polyphosphate kinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only