Addgene

We narrowed to 2,387 results for: MEA;

Showing: 441 - 460 of 2387 results

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PX458_ZNF580_1

Plasmid

#86331

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_2

Plasmid

#86313

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SAP130_2

Plasmid

#86311

Purpose

Encodes gRNA for 3' target of human SAP130

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SAP130 (SAP130 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZSCAN9

Plasmid

#86268

Purpose

Donor vector for 3' FLAG tag of human ZSCAN9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZSCAN9 homology arms (ZNF193 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pNB762

Plasmid

#60150

Purpose

Firefly luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB767

Plasmid

#60159

Purpose

Firefly luciferase (Promega) driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

SIC1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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Phamret-H2B/pcDNA3

Plasmid

#51955

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to H2B

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Use

Tags

human H2B

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Phamret-Fibrillarin/pcDNA3

Plasmid

#51954

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Use

Tags

Fibrillarin

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pET12a-PAPI

Plasmid

#19961

Depositor

Jonathan Tait

Article

Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Insert

Annexin V (ANXA5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pSIRV-NFAT-eGFP

Plasmid

#118031

Purpose

Fluorescent reporter for NFAT activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

minimal promoter with NFAT binding site

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

minimal promoter with NF-kB responsive element

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSIRV-AP-1-mCherry

Plasmid

#118095

Purpose

Fluorescent reporter for AP-1 activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

mCherry

Use

Retroviral

Tags

Expression

Mutation

Promoter

minimal promoter with AP-1 responsive element

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205550

Purpose

Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.12_GagZip-MCP-P2A-eGFP

Plasmid

#205527

Purpose

Express HIV GagZip-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP

Use

Tags

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205546

Purpose

Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFH3.3_Cre-MS2x12

Plasmid

#205559

Purpose

Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRClover

Plasmid

#59150

Purpose

Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-DEVD-NanoLuc

Plasmid

#98287

Purpose

caspase-3 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pRPR1(TetO)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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