We narrowed to 849 results for: nanobody

1. pET29b_GST-NbD6

   Plasmid

   #228896

   Purpose

   Express GST-tagged anti-Zika NbD6 in E.coli

   Depositor

   Magnus Rueping

   Article

   Yuli et al ACS Synthetic Biology Article ASAP

   Insert

   GST-tagged NbD6
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEx411c_pelB-NbD6-Strep2

   Plasmid

   #228897

   Purpose

   Periplasmic Expresssion of Strep-tagged anti-Zika NbD6

   Depositor

   Stefan Arold

   Article

   Yuli et al ACS Synthetic Biology Article ASAP

   Insert

   sb0379
   

   Use

   Tags

   Strep2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEx411c_pelB-N32-Strep2

   Plasmid

   #228898

   Purpose

   Periplasmic Expresssion of Strep-tagged anti-Zika NbD32

   Depositor

   Stefan Arold

   Article

   Yuli et al ACS Synthetic Biology Article ASAP

   Insert

   sb0380
   

   Use

   Tags

   Strep2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCOLA-Gent-EV

   Plasmid

   #202491

   Purpose

   pCOLA-Gent Empty Vector

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sybody Generation Toolbox

   Plasmid Kit

   #1000000160

   Purpose

   The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.

   Depositor

   Markus Seeger

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

6. pTrcHIS-wt7D12

   Plasmid

   #125268

   Purpose

   Expresses wild type 7D12 anti-EGFR nanobodies

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 nanobody
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Fab_8D3_2_L

   Plasmid

   #176076

   Purpose

   Express light chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   Light chain of Fab_8D3_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. prhaBAD-anti-rabbit-Nb-Hia5

   Plasmid

   #239627

   Purpose

   Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

   Depositor

   Aaron Straight

   Article

   Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

   Insert

   Hia5
   

   Use

   Tags

   6HIS, MBP, TEV, and anti-Rabbit Nanobody

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   rhaBAD

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. iMbody_pSANG10-3F

   Plasmid

   #184496

   Purpose

   Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

   Depositor

   Marcel Dinger

   Article

   Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

   Insert

   iMbody
   

   Use

   Tags

   3xFLAG, 6xHis, and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCOLA-Spec-EV

    Plasmid

    #212095

    Purpose

    (Empty Backbone) pCOLA-Spec Empty Vector

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2 PCNA GFP

    Plasmid

    #105937

    Purpose

    in vitro transcription, cell cycle nuclear marker

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-APEX2

    Plasmid

    #108894

    Purpose

    Multisite gateway vector for 3' tagging with APEX2

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    APEX2
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-mCherry

    Plasmid

    #108884

    Purpose

    Multisite gateway vector for 3' tagging with mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    mCherry
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-P-RAN-GFP26

    Plasmid

    #106406

    Purpose

    P-RAN-GFP26 (RANbody GFP26::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-GFP26
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIREShygro HA-mVenus-mAID

    Plasmid

    #117714

    Purpose

    Expression of HA-tagged mVenus fused to mAID

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mVenus-mAID
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Y-RAN-GFP26

    Plasmid

    #106423

    Purpose

    Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-GFP26
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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