We narrowed to 1,008 results for: nanobody

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Actin-OptoNB

Plasmid

#159595

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Publication

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

ActinNB with AsLOV2 at loop 5 with 62-666

Use

Lentiviral

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

Plasmid

#128268

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

Depositor

Stephen Royle

Publication

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(III)-GBPen

Expression

Mammalian

Promoter

CMV and IRES

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP-P2A-APEX2-csChBP

Expression

Mammalian

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP1

Plasmid

#106422

Purpose

Y-RAN-GFP1 (RANbody GFP1::Chicken IgY)

Depositor

Joshua Sanes

Publication

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMito-GBPen

Plasmid

#128269

Purpose

Expression of mitochondrially targeted GBPen (GFP Nanobody)

Depositor

Stephen Royle

Publication

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Insert

Mito-GBPen

Expression

Mammalian

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pETNb-cALFA

Plasmid

#159989

Purpose

For the protein expression with ALFA tag at C-terminus in E. coli

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Bacterial

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Gateway Cloning

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Publication

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP4

Plasmid

#106410

Purpose

P-RAN-RFP4 (RANbody RFP4::vHRP)

Depositor

Joshua Sanes

Publication

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP4

Tags

HA and His

Expression

Mammalian

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Publication

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET21b-RBD-JA1

Plasmid

#167126

Purpose

Expresses SARS-CoV-2 RBD in bacterial system

Depositor

David Margulies

Publication

Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

Insert

SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)

Expression

Bacterial

Mutation

residues (333 to 529 aa)

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-17

Plasmid

#172747

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-17

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-29

Plasmid

#172753

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-29

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-1

Plasmid

#172767

Purpose

Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-1

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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