We narrowed to 2,320 results for: pcas9

1. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-MP-84-spCas9-U6-gRNA-tlock

   Plasmid

   #206936

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   MP-84

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

   Plasmid

   #148897

   Purpose

   Mammalian Expression of HsHelz-sgRNA

   Depositor

   Elisa Izaurralde

   Article

   Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

   Insert

   HsHelz-sgRNA (HELZ Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

   Plasmid

   #148848

   Purpose

   Mammalian Expression of HsAGO2-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO2-sgRNA1 (AGO2 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

   Plasmid

   #148849

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

    Plasmid

    #148850

    Purpose

    Mammalian Expression of HsTNRC6B-sgRNA1

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-sgRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

    Plasmid

    #148851

    Purpose

    Mammalian Expression of HsAGO2-sgRNA2

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO2-sgRNA2 (AGO2 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

    Plasmid

    #148852

    Purpose

    Mammalian Expression of HsTNRC6A-sgRNA2

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6A-sgRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

    Plasmid

    #148853

    Purpose

    Mammalian Expression of HsTNRC6B-sgRNA3

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-sgRNA3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

    Plasmid

    #148854

    Purpose

    Mammalian Expression of HsAGO1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO1-sgRNA (AGO1 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

    Plasmid

    #148856

    Purpose

    Mammalian Expression of HsNot1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1-sgRNA (CNOT1 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

    Plasmid

    #148857

    Purpose

    Mammalian Expression of HsPatL1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsPatL1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

    Plasmid

    #148858

    Purpose

    Mammalian Expression of HsTNRc6C-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRc6C-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

    Plasmid

    #141049

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF4-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge