Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,836 results for: pcas9

Clear keywords
Showing: 441 - 460 of 4836 results

  2. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    208829-plasmid-map-sequence-id-419661

    Enlarge

  4. pX461-ABE8e-nSpCas9

    Plasmid
    #237460
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237460-plasmid-map-sequence-id-476159

    Enlarge

  5. pX461-evoCDA1-nSpCas9

    Plasmid
    #237461
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237461-plasmid-map-sequence-id-476160

    Enlarge

  9. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

    Enlarge

  11. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

    Enlarge

  13. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

    Enlarge

  16. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209042-plasmid-map-sequence-id-413615

    Enlarge

  20. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available Since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

    Enlarge

Showing: 441 - 460 of 4836 results