We narrowed to 2,365 results for: pcas9

1. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

   Plasmid

   #211821

   Purpose

   PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter, U6 promoter and hPGK pr…

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-MP-84-spCas9-U6-gRNA-tlock

   Plasmid

   #206936

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   MP-84

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

   Plasmid

   #148897

   Purpose

   Mammalian Expression of HsHelz-sgRNA

   Depositor

   Elisa Izaurralde

   Article

   Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

   Insert

   HsHelz-sgRNA (HELZ Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

   Plasmid

   #148848

   Purpose

   Mammalian Expression of HsAGO2-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO2-sgRNA1 (AGO2 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

   Plasmid

   #148849

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

    Plasmid

    #148850

    Purpose

    Mammalian Expression of HsTNRC6B-sgRNA1

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-sgRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

    Plasmid

    #148851

    Purpose

    Mammalian Expression of HsAGO2-sgRNA2

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO2-sgRNA2 (AGO2 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

    Plasmid

    #148852

    Purpose

    Mammalian Expression of HsTNRC6A-sgRNA2

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6A-sgRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

    Plasmid

    #148853

    Purpose

    Mammalian Expression of HsTNRC6B-sgRNA3

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-sgRNA3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

    Plasmid

    #148854

    Purpose

    Mammalian Expression of HsAGO1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO1-sgRNA (AGO1 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

    Plasmid

    #148856

    Purpose

    Mammalian Expression of HsNot1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1-sgRNA (CNOT1 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

    Plasmid

    #148857

    Purpose

    Mammalian Expression of HsPatL1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsPatL1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

    Plasmid

    #148858

    Purpose

    Mammalian Expression of HsTNRc6C-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRc6C-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

    Plasmid

    #141049

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF4-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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