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pAAV-EF1a-ArgiNLS-mGreenLantern

Plasmid

#220596

Purpose

Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mGreenLantern

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP1

Plasmid

#129504

Purpose

Expresses AvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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phage UbiC SNAP-DDX6

Plasmid

#119948

Purpose

Lentiviral vector expressing SNAP-DDX6 fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

SNAP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP2

Plasmid

#129505

Purpose

Expresses AvicFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

Plasmid

#216491

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

NES, T2A-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-PHIP

Plasmid

#65389

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PHIP (PHIP Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_short (JDW 1046)

Plasmid

#224480

Purpose

Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

min-ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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ER-mGreenLantern

Plasmid

#164463

Purpose

Localization and retention of mGreenLantern fluorescent protein in the ER

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DDX54

Plasmid

#97060

Purpose

Expresses FLAG/HA-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only
pHAGE-FLAG-APEX2-MAP1LC3BK51A

Plasmid

#175754

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Mutation

K51A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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VAMP8-mCherry

Plasmid

#92424

Purpose

Expression of VAMP8 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP8 (Vamp8 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

A36S (Please see Depositor Comments below)

Promoter

CMV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Article

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Tags

Venus

Expression

Mammalian

Promoter

metallothionein promoter

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

Plasmid

#74309

Purpose

Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-EGFP

Plasmid

#220594

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND11

Plasmid

#65402

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND11 (ZMYND11 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

Plasmid

#100846

Purpose

AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-PKIalpha-IRES-nls-mRuby2

Plasmid

#63059

Purpose

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

PKIalpha (Pkia Synthetic, Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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