We narrowed to 4,712 results for: lum

1. pUC19-A7HP89

   Plasmid

   #103082

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   A7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEP-LAMP1-RFP

   Plasmid

   #167970

   Purpose

   Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

   Depositor

   Juan Bonifacino

   Article

   Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   SEP and mRFP

   Expression

   Mammalian

   Mutation

   D50E

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

   Plasmid

   #176027

   Purpose

   construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   rtTA3
   Luc2
   mtagBFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG RpH-LAMP1-3xFLAG

   Plasmid

   #163018

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG R2pH-LAMP1-3xFLAG

   Plasmid

   #157940

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Tags

   3XFLAG, mCherry, and pHluorin

   Expression

   Mammalian

   Promoter

   Chick beta actin pomoter

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEntr_Myc-BirA-G3-ER

   Plasmid

   #173814

   Purpose

   Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

   Depositor

   Norbert Perrimon

   Article

   Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

   Insert

   Myc-BirA-G3-ER
   

   Use

   Gateway shuttle vector

   Tags

   Myc

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NanoLuc-PM

   Plasmid

   #164784

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
   

   Tags

   NanoLuc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIBN-EYFP-TetR

   Plasmid

   #103798

   Purpose

   BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-TetR (CIB1, tetR Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIBN-EYFP-hPMLIII

   Plasmid

   #103808

   Purpose

   BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. GLuc-PM

    Plasmid

    #164783

    Purpose

    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    

    Tags

    GLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-FLAG-TAPBPR

    Plasmid

    #135500

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Tags

    Luminal FLAG epitope tag and Signal peptide from …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pStr-KDEL_TPV-CXCL9-mCherry-SBP

    Plasmid

    #170677

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    CXCL9 (CXCL9 Human, Synthetic)
    streptavidin-KDEL
    

    Tags

    SBP and mCherry

    Expression

    Mammalian

    Promoter

    IRES and T7

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. WP_010880027.1

    Plasmid

    #194729

    Purpose

    Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

    Depositor

    Fang Li

    Article

    Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

    Insert

    nanoparticle-proteinA and His tag
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC590 - pCMV ER-iRFP

    Plasmid

    #59136

    Purpose

    A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    ER-localized Infrared Fluorescent Protein
    

    Tags

    ER retention signal and signal peptide

    Expression

    Mammalian

    Promoter

    CMV-IE

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniSOG-ER-5

    Plasmid

    #57766

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    ER (CALR Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDONOR223 RpH-LAMP1-3xFLAG

    Plasmid

    #163019

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-STIM1-NN_neomycin

    Plasmid

    #65311

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIBN-tagBFP-hTRF2

    Plasmid

    #103803

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-STIM1-NN_puromycin

    Plasmid

    #65310

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge