We narrowed to 17,149 results for: pcDNA

1. pCDNA3 Flag MKK7b1(glu)

   Plasmid

   #14673

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7b1(Glu) (Map2k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-269Glu Thr-273Glu

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3 Flag MKK3b(Glu)

   Plasmid

   #50449

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 218 to Glutamic Acid, changed Thre…

   Promoter

   CMV

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO-AT1R-APEX

   Plasmid

   #96847

   Purpose

   For expression of AT1R-APEX in mammalian cells

   Depositor

   Andrew Kruse

   Article

   Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

   Insert

   AT1R-APEX (AGTR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-MTS-CA-c-Src-FLAG

   Plasmid

   #44654

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed Tyr 530 to Phe

   Promoter

   CMV promoter

   Available since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-HA-Akt1-aa1-149

   Plasmid

   #73410

   Purpose

   Expresses HA tagged aa1-149 of AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 1-149 only of AKT1

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Yellow Cameleon-Nano50/pcDNA3

   Plasmid

   #51964

   Purpose

   mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano50
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO-FNTA-FLAG

   Plasmid

   #86858

   Purpose

   To express FNTA with a FLAG tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTA (FNTA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The sequence of the insert is isoform 2, which di…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Flag-Tet3 CD Mut

   Plasmid

   #72222

   Purpose

   Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 3
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET3 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-2xFLAG-2xSTREP-CDK6

   Plasmid

   #172614

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 CMV NAPA-A BGH

    Plasmid

    #92277

    Purpose

    FRET probe; extracellular membrane tethered eGFP

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Astrocyte
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/GUG-nLuc-3XFLAG

    Plasmid

    #127301

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid

    #112494

    Purpose

    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of DD domain (AA584-671 deleted)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Use

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. integrin beta6 pcDNA1 neo

    Plasmid

    #13580

    Depositor

    Dean Sheppard

    Article

    Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/TO-myc-BirA*-NES

    Plasmid

    #112714

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    HIV Rev nuclear export signal (NES)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MYO10-HMM-Nanotrap

    Plasmid

    #87255

    Purpose

    Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    GFP-nanotrap

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT-ChemoD-NAD

    Plasmid

    #193826

    Purpose

    CMV overexpression of ChemoD-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoD-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ShadowG: T225R, HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

    Plasmid

    #115789

    Purpose

    Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    NDP (NDP Human)
    

    Use

    Tags

    alkaline phosphatase and flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-FLAG-SHLD3

    Plasmid

    #114123

    Purpose

    Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge