We narrowed to 16,609 results for: IGH@
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Plasmid#109863PurposeProtein expression and purification of EPHB4_Ephrin-lbdDepositorInsertEPHB4_Ephrin-lbd (EPHB4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KPNA2_Arm
Plasmid#109866PurposeProtein expression and purification of KPNA2_ArmDepositorInsertKPNA2_Arm (KPNA2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABCE1_ABC-tran
Plasmid#109853PurposeProtein expression and purification of ABCE1_ABC-tranDepositorInsertABCE1_ABC-tran (ABCE1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EIF2S3_GTP-EFTU
Plasmid#109829PurposeProtein expression and purification of EIF2S3_GTP-EFTUDepositorInsertEIF2S3_GTP-EFTU (EIF2S3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARPC5_P16-Arc
Plasmid#109802PurposeProtein expression and purification of ARPC5_P16-ArcDepositorInsertARPC5_P16-Arc (ARPC5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBA2_ThiF
Plasmid#109787PurposeProtein expression and purification of UBA2_ThiFDepositorInsertUBA2_ThiF (UBA2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARPC3_P21-Arc
Plasmid#109793PurposeProtein expression and purification of ARPC3_P21-ArcDepositorInsertARPC3_P21-Arc (ARPC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT140_GalL_cCDA1-VRERBE3
Plasmid#145103PurposeExpressing base editor cCDA1-VRERBE3 in yeast cellsDepositorInsertcCDA1-VRERBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)
Plasmid#153439PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT176_GalL_eA3A-BE3
Plasmid#145125PurposeExpressing base editor eA3A-BE3 in yeast cellsDepositorInsertA3A(N57G)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(N57G); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT174_GalL_A3A-Y130F-BE3
Plasmid#145123PurposeExpressing base editor A3A-Y130F-BE3 in yeast cellsDepositorInsertA3A(Y130F)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(Y130F); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT173_GalL_A3A-R128A-BE3
Plasmid#145122PurposeExpressing base editor A3A-R128A-BE3 in yeast cellsDepositorInsertA3A(R128A)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(R128A); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT122_GalL_cCDA1-VQRBE3
Plasmid#145087PurposeExpressing base editor cCDA1-VQRBE3 in yeast cellsDepositorInsertcCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT113_GalL_cCDA1-xBE3
Plasmid#145079PurposeExpressing base editor cCDA1-xBE3 in yeast cellsDepositorInsertcCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:AgBIS:Flag
Plasmid#133971PurposeHeterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.DepositorInsertAgBIS:Flag
UseSynthetic BiologyTagsFlagExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:MrBBS:Strep
Plasmid#133973PurposeHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.DepositorInsertMrBBS:StrepII
UseSynthetic BiologyTagsStrepIIExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph20-Stg
Plasmid#133035PurposeExpresses Xph20-Stg in bacteriaDepositorInsertXph20-Stg
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-ETWV
Plasmid#133036PurposeExpresses Xph18-ETWV in bacteriaDepositorInsertXph18-ETWV
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-Stg
Plasmid#133037PurposeExpresses Xph18-Stg in bacteriaDepositorInsertXph18-Stg
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph15-ETWV
Plasmid#133038PurposeExpresses Xph15-ETWV in bacteriaDepositorInsertXph15-ETWV
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph0-ETWV
Plasmid#133039PurposeExpresses Xph0-ETWV in bacteriaDepositorInsertXph0-ETWV
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-TAT-Xph20-ETWV
Plasmid#133040PurposeExpresses TAT-Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
UseTagsHis8, TAT, and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed HEL-binding scFv D44.1des
Plasmid#111719PurposeYeast Surface Display of D44.1desDepositorInsertD44.1 des
UseTagscmyc tagExpressionYeastMutationL Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…PromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5
Plasmid#117137PurposeExpression vector encoding BFP-BSD-Nkx2-5DepositorInsertNkx2-5 CDS (Nkx2-5 Mouse)
UseLentiviralTagsExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3038
Plasmid#122545PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_glo-1::CemOrange2 (glo-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::lgg-1 (lgg-1 Nematode, Synthetic)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-HF2-BE3
Plasmid#120397PurposeExpresses HF2-BE3 in mammalian cellsDepositorInsertHF2-BE3
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
PME-1_R369D mutant
Plasmid#119294PurposeMammalian overexpression of StrepII-tagged full length PPME1 with mutation in the PP2Ac binding motifDepositorInsertPPME1 (PPME1 Human)
UseTagsExpressionMammalianMutationR369DPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-PAmCherry-cPHx1
Plasmid#116859PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsPAmCherryExpressionMammalianMutationamino acids 169-329PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ATP5
Plasmid#109857PurposeProtein expression and purification of ATP5DepositorInsertATP5 (ATP5J Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
UseTags6His-TEVExpressionBacterialMutationR47APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
UseTags6His-TEVExpressionBacterialMutationR47KPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
UseTags6His-TEVExpressionBacterialMutationQ48APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
UseTags6His-TEVExpressionBacterialMutationT50APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
UseTags6His-TEVExpressionBacterialMutationR52APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(delta C-term)
Plasmid#112731PurposeThis mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.DepositorInsert6His-TEV-TBP6.7(delta C-term)
UseTags6His-TEVExpressionBacterialMutationdelta C-termPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2+IA
Plasmid#111273Purposeexpress murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-shIl22ra2-1408
Plasmid#99032PurposeExpresses a shRNA targeting the rat Il22ra2 geneDepositorInsertIl22ra2 (Il22ra2 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-shIl22ra2-1928
Plasmid#99033PurposeExpresses a shRNA targeting the rat Il22ra2 geneDepositorInsertIl22ra2 (Il22ra2 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSM4
Plasmid#101642PurposeEncodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.DepositorInsertKCyG4219
UseTags6XHisExpressionBacterialMutationC-terminal residues (221-223) deletion. Ser220Val…PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10, RGE)-I27-CC
Plasmid#85396PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationGRGDS motif mutated to GRGESPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-H2B-C-10
Plasmid#57789PurposeLocalization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertH2B (H2BC11 Human)
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-874-Decoy.hPGK.GFP.WPRE
Plasmid#46637DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-330-Decoy.hPGK.GFP.WPRE
Plasmid#46611DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-744-Decoy.hPGK.GFP.WPRE
Plasmid#46633DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-195-Decoy.hPGK.GFP.WPRE
Plasmid#46598DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL2
Plasmid#41836DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hsyn-GRAB_DA2h (AAV9)
Viral Prep#140554-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2h (#140554). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2h plasmid DNA. Synapsin-driven expression of high-affinity GRAB_DA2h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1h (AAV9)
Viral Prep#140557-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1h (#140557). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1h plasmid DNA. Synapsin-driven expression of red fluorescent, high affinity dopamine sensor GRAB_rDA1h. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only