We narrowed to 4,859 results for: U6...

1. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

   Plasmid

   #91879

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

2. px458-CCND1

   Plasmid

   #172630

   Purpose

   Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against CCND1 (CCND1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

   Plasmid

   #161923

   Purpose

   To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting HSD17B11 exon 1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTEE

   Plasmid

   #159748

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX335 HTT sgRNA-a

   Plasmid

   #87201

   Purpose

   pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   HTT (HTT Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX335 HTT sgRNA-b

   Plasmid

   #87200

   Purpose

   pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   HTT (HTT Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

   Plasmid

   #204852

   Purpose

   AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSaCas9KKH(D10A)-UGI
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   D10A, E782K, N968K, R1015H in SaCas9

   Promoter

   EFS, miniU6

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

   Plasmid

   #91880

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.puro_shGLS

   Plasmid

   #110335

   Purpose

   shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Article

   Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

   Insert

   GLS glutaminase (GLS Human, Synthetic)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA PolIII)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2_mClover3_AAVS1_sgRNA_2

    Plasmid

    #155099

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LCV2_AAVS1_sgRNA_3

    Plasmid

    #155089

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LCV2_PSMB2_sgRNA_1

    Plasmid

    #155092

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMB2_sgRNA_1 (PSMB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LCV2_PSMD1_sgRNA_1

    Plasmid

    #155091

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LCV2_mCherry_PSMD1_sgRNA_1

    Plasmid

    #155107

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEE

    Plasmid

    #159746

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLL5.0 shCavin1KDR-EGFP

    Plasmid

    #187254

    Purpose

    Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

    Depositor

    Alpha Yap

    Article

    Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

    Insert

    ShRNA for Cavin1 (Cavin1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6, 5'LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9-hGeminin

    Plasmid

    #199344

    Purpose

    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

    Depositor

    Iain Hagan

    Article

    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

    Insert

    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    

    Use

    Tags

    cas9 c-term fused to hgemenin 1-110 fragment

    Expression

    Mammalian

    Mutation

    cas9 c-term fused to hgemenin 1-110 fragment

    Promoter

    cbh

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HEK4-53bp-tag-insertion-pegRNA

    Plasmid

    #227673

    Purpose

    PegRNA for 53 bp insertion

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    SpCas9 sgRNA scaffold (EPHA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RB1-TP53-LRG-GFP

    Plasmid

    #225876

    Purpose

    Guides targeting TP53 and RB1

    Depositor

    Janai Carr-Ascher

    Article

    Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

    Insert

    RB1 and TP53 (TP53, RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-sgBrip1#2/Cre

    Plasmid

    #193204

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgBrip1#2 (Brip1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge