We narrowed to 13,403 results for: mpl

1. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTX1-SMN2-exon7-ESE1

   Plasmid

   #126042

   Purpose

   In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   SMN2 exon7 ESE1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

   Plasmid

   #157674

   Purpose

   Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L-His-SpyTag-XMod(XL2V)-Doc

   Plasmid

   #157676

   Purpose

   Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   XMod-Doc mutant XL2V with Histag and SpyTag
   

   Use

   Tags

   Histag and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2365 - Intron GG1 - 250bp no PATC

   Plasmid

   #159883

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 250bp no PATC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2345 - Intron GG2 - 250bp no PATC

   Plasmid

   #159884

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - 250bp no PATC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ2366 - intron GG3 - 250bp no PATC

   Plasmid

   #159885

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG3 - 250bp no PATC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSWD95

   Plasmid

   #158617

   Purpose

   CckA FRET Sensor

   Depositor

   Seth Childers

   Article

   Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

   Insert

   CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Xylose

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET_4E1H.95

   Plasmid

   #155213

   Purpose

   Expresses 4E1H.95 de novo protein in E.coli

   Depositor

   Bruno Correia

   Article

   Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

   Insert

   4E1H.95
   

   Use

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET_4H.01

    Plasmid

    #155210

    Purpose

    Expresses 4H.01 de novo designed protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4H.01
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4E1H.95_LUMABS

    Plasmid

    #155208

    Purpose

    Expresses 4E1H.95_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E1H.95_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET_3E2H.37

    Plasmid

    #155211

    Purpose

    Expresses 3E2H.37 de novo designed protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3E2H.37
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET_3H1L_02.395

    Plasmid

    #155198

    Purpose

    Expresses 3H1L_02.395 in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3H1L_02.395
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCk4

    Plasmid

    #136698

    Purpose

    Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pst39-NST-clone14

    Plasmid

    #133421

    Purpose

    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

    Depositor

    Gerhard Wagner

    Insert

    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ORF for Nip1 encodes aa 4-156 (not complete ORF),…

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LC8-like

    Plasmid

    #133115

    Purpose

    Human LC8-like protein, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    LC8-like (LOC392067 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hy_pMtnA UTR delta 21-55 eGFP SV40

    Plasmid

    #132653

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hy_pMtnA UTR delta 41-55 eGFP SV40

    Plasmid

    #132652

    Purpose

    Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMaCTag-Z19

    Plasmid

    #124791

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. YSBE420:pNEO_CAC1_Vn_HOG1 terminator

    Plasmid

    #122701

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    N-terminal fragment of Venus protein
    

    Use

    Tagging proteins in cryptococcus neoformans

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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