Addgene

We narrowed to 23,361 results for: Nov;

Showing: 6931 - 6960 of 23361 results

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pET SUMO-cPLA2 C2 domain (Y96C)

Plasmid

#213633

Purpose

Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

cPLA2 C2 domain (Y96C) (PLA2G4A Human)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Y96C, C139A, C141S

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45

Plasmid

#208796

Purpose

Xenopus laevis FLAG5-His10-TEV-Cdc45

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

Cdc45 (cdc45.L Frog)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-WM

Plasmid

#208798

Purpose

Xenopus laevis His6-Myc-DONSON (W234L, M463T)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

W234L; M463T

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-3A

Plasmid

#208800

Purpose

Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

V473A; R476A; Y481A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-ΔD1

Plasmid

#208804

Purpose

Xenopus laevis His6-DONSON (1-155aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His

Expression

Bacterial

Mutation

ΔD1, deletion of 1-155aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115G)-Talin rod-mCherry

Plasmid

#210026

Purpose

Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry

Use

Tags

Expression

Mammalian

Mutation

SpyTag003 M115G mutation to modify SpyCatcher003 …

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115A)-Talin rod-mCherry

Plasmid

#210025

Purpose

Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry

Use

Tags

Expression

Mammalian

Mutation

SpyTag003 M115A mutation to modify SpyCatcher003 …

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-GEMS(JAK-STAT)

Plasmid

#209109

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-GEMS(JAK-STAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Use

Tags

Expression

Bacterial

Mutation

A28C in the I-F repeat

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-L1-G1397N

Plasmid

#210396

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-L1-G1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-H2-G1397C

Plasmid

#210395

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-H2-G1397C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-G'-l2-G'

Plasmid

#209120

Purpose

Bacterial expression of CC-GEMS ligand SUMO-G'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-G'-l2-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a3-GEMS(JAK-STAT)

Plasmid

#209111

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a3-GEMS(JAK-STAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 IntS12

Plasmid

#210521

Purpose

Expresses GST-tagged Drosophila IntS12

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS12 (IntS12 Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 DomI + DomII pMT2

Plasmid

#206095

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tag

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l2-A'-IRES-EmGFP

Plasmid

#209125

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-449 POLH

Plasmid

#201583

Purpose

Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

POLH (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-449 of DNA polymerase eta

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLI

Plasmid

#201440

Purpose

Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJM919_WT His POLI

Plasmid

#201444

Purpose

Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

lacIq

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11A(E186Q)-HA

Plasmid

#209261

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E186Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B4(E170Q)-HA

Plasmid

#209255

Purpose

Mammalian expression of ATP8B4 mutant E170Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B4 (ATP8B4 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E170Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B4(E170Q)-HA

Plasmid

#209243

Purpose

Mammalian expression of ATP8B4 mutant E170Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B4 (ATP8B4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E170Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B1-HA

Plasmid

#209226

Purpose

Mammalian expression of ATP8B1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge