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Showing: 6931 - 6960 of 48991 results

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pAT9784-xABE

Plasmid

#163005

Purpose

plasmid expressing xABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

xABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2U2T2-YFP

Plasmid

#84550

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2U2T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2T2U2-YFP

Plasmid

#84552

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2T2U2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

Plasmid

#157674

Purpose

Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

SpyCatcher with ddFLN4, ELP, Histag and ybbR tag

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod(XL2V)-Doc

Plasmid

#157676

Purpose

Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc mutant XL2V with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-no charge

Plasmid

#160532

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, E337A, N338A, K341A, N342A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-no charge

Plasmid

#160525

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, E337A, N338A, K341A, N342A

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAflaB

Plasmid

#149561

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNArodA

Plasmid

#149569

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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R2x2_VAL88

Plasmid

#160761

Purpose

Expresses R2x2_VAL88 in bacterial cells

Depositor

Nobuyasu Koga

Article

Koga et al Proc Natl Acad Sci U S A. 2020 Nov 23. p

Insert

R2x2_VAL88

Tags

6xHis

Expression

Bacterial

Mutation

10-residue mutations of Rsmn2x2_5_6

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0826S-sgRNA500

Plasmid

#149618

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0826S

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTL-sgRNA500

Plasmid

#149617

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pXW101Plux2-Pecf11-gfp

Plasmid

#160823

Purpose

Two-layered AHL-responsive circuit carrying an ECF11-based amplifier

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23101, Plux2, Pecf11

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNA500

Plasmid

#149559

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - L39V)

Plasmid

#136607

Purpose

Expresses DANCER-3 mutant (reversion L39V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (L39V reversion)

Tags

5x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I54V)

Plasmid

#136608

Purpose

Expresses DANCER-3 mutant (reversion I54V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I54V reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F34A)

Plasmid

#136606

Purpose

Expresses DANCER-3 mutant (reversion F34A)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F34A reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I7L)

Plasmid

#136605

Purpose

Expresses DANCER-3 mutant (reversion I7L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I7L reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F3Y)

Plasmid

#136604

Purpose

Expresses DANCER-3 mutant (reversion F3Y)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F3Y reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNAmreB

Plasmid

#149634

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNArodA

Plasmid

#149635

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_Psyn-sgRNA500

Plasmid

#149614

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTS-sgRNA500

Plasmid

#149616

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0826L-sgRNA500

Plasmid

#149619

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0826L

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0526-sgRNA500

Plasmid

#149621

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0031-sgRNA500

Plasmid

#149622

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0031

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaBS-sgRNAflaB

Plasmid

#149623

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaBS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTS-sgRNAflaB

Plasmid

#149624

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PresTL-sgRNAflaB

Plasmid

#149625

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge