We narrowed to 5,260 results for: PID

1. pET22-His-TEV-DOT1L-MM

   Plasmid

   #89611

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L VVEL293MM Mutant (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXS-IRES-Blast ACSL4

   Plasmid

   #173164

   Purpose

   Retroviral vector to express sgRNA resistant human ACSL4

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

   Plasmid

   #161923

   Purpose

   To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting HSD17B11 exon 1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pdCas9-M-G6

   Plasmid

   #73437

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor G6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXS-IRES-Blast 3XFLAG-CHP1

   Plasmid

   #173166

   Purpose

   Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
   

   Use

   Retroviral

   Tags

   3XFLAG

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pdCas9-M-3A2

   Plasmid

   #73435

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 3A2 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLX304_C12orf49-V5

   Plasmid

   #155110

   Purpose

   lentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LCV2_mClover3_AAVS1_sgRNA_2

   Plasmid

   #155099

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pdCas9-M-4A6

    Plasmid

    #73434

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4A6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMBP-parallel1-GK5_v2

    Plasmid

    #134288

    Purpose

    Expresses GK5 (isoform 2) in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PTRF/Cavin1_L (OZ513)

    Plasmid

    #27188

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pGL3 2198

    Plasmid

    #49347

    Purpose

    This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.

    Depositor

    Stephen Harris, Melissa Rogers

    Article

    Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1)

    Insert

    Bmp2 promoter (shorter sequence) (BMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Bmp2 promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5 FLAG membrane-bound lipin-1 (TMD)

    Plasmid

    #120277

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG and in-frame fusion with the TMD Golgi-local…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mTagBFP2-FKBP-CYB5Atail

    Plasmid

    #108141

    Purpose

    For recruitment of ER

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mTagBFP2:FKBP1A(3-108):[GGSA]4GG:CYB5A(100-134) (CYB5A, FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CYB5A(100-134)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pdCas9-M-C4

    Plasmid

    #73427

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor C4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdCas9-M-3F2

    Plasmid

    #73428

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor C4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LCV2_AAVS1_sgRNA_3

    Plasmid

    #155089

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LCV2_AAVS1_sgRNA_2

    Plasmid

    #155088

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge