We narrowed to 7,489 results for: addgene/1000
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Plasmid#218522PurposesgRNA targeting human FLVCR1DepositorInsertFLVCR1 (FLVCR1 Human)
UseCRISPR and LentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro CHKA_sg2
Plasmid#218523PurposesgRNA targeting human CHKADepositorInsertCHKA (CHKA Human)
UseCRISPR and LentiviralAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v1 GFP FLVCR1_sg5
Plasmid#218521PurposesgRNA targeting human FLVCR1DepositorInsertFLVCR1 (FLVCR1 Human)
UseCRISPR and LentiviralAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR1A-DuET
Plasmid#213316PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR119-DuET
Plasmid#213259PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCKAR-DuET
Plasmid#213195PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ALX4-Flag
Plasmid#215029PurposeMammalian expression of human ALX4DepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_ALX4-Flag-HA
Plasmid#215028PurposeMammalian expression of human ALX4DepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR
Plasmid#207136PurposeDNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-inDepositorInsert5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
ExpressionMammalianAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 DomI + DomII pMT2
Plasmid#206095Purposeexpression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tagDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A pMT2
Plasmid#206110Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin bindingDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 R57A, R59A pMT2
Plasmid#206112Purposeexpression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulationDepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 D122A pcDNA3
Plasmid#206068Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1DepositorAvailable SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 HA D491A pcDNA3
Plasmid#206070Purposeexpression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin bindingDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPGN
Plasmid#199232PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpithelial mitogen isoform 6 (EPGN Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tev-IntC-P53(304-393)
Plasmid#200315Purposebacterial expression of P53 TETCTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6GB1tev and designed intein CExpressionBacterialPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-ATG9A-N
Plasmid#198530PurposeExpression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastPromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FHIP-L
Plasmid#198528PurposeExpression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)DepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH
Plasmid#148878PurposeMammalian Expression of TNFalpha-ARE-polyA-MalatDepositorInsertTNFalpha-ARE-polyA-Malat (TNF Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only