We narrowed to 12,296 results for: ski

1. pCAG-Cre-T2A-mRuby2

   Plasmid

   #102989

   Purpose

   Expresses Cre and mRuby2

   Depositor

   Karin Scharffetter-Kochanek

   Article

   Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

   Inserts

   Cre (site-specific recombinase)
   mRuby2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-HypocratesCS

   Plasmid

   #161960

   Purpose

   Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   Hypocrates
   

   Expression

   Mammalian

   Mutation

   changed Cysteine 355 to Serine

   Promoter

   CMV, SP6

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. JpExpress404 PTEN-LONG

   Plasmid

   #49417

   Purpose

   Bacterial Expression Vector

   Depositor

   Ramon Parsons

   Article

   Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

   Insert

   PTEN-Long (PTEN )
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-His6-LOXCAT

   Plasmid

   #140084

   Purpose

   Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV/eGFP-U6-sgRNA

   Plasmid

   #170544

   Purpose

   A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hchk2 (kin-)-PUNI10

   Plasmid

   #41908

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   CHK2 (CHEK2 Human)
   

   Expression

   Bacterial

   Mutation

   kinase dead (D347A)

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TRBV7-9

   Plasmid

   #123437

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV7-9 (TRBV7-9 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRAV26-1

   Plasmid

   #123398

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV26-1 (TRAV26-1 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SNAP-Drp1

    Plasmid

    #141159

    Purpose

    Mammalian expression and labeling of Drp1

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    DRP1 (DNM1L Human)
    

    Tags

    SNAP-Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-Nsp16-HF

    Plasmid

    #157725

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16-HF (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA5-FRT-TO-Nsp16

    Plasmid

    #157723

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGEX6P1-HA-MEK1

    Plasmid

    #53159

    Purpose

    Expresses GST-HA tagged human MEK1 from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBeta-lacZ-attBx2

    Plasmid

    #124445

    Purpose

    Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Type

    Empty backbone

    Use

    Enhancer reporter assay construct

    Tags

    LacZ

    Expression

    Mammalian

    Promoter

    beta globin minimal promoter

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX SHP-1(NC)-SH2

    Plasmid

    #46496

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(NC) (PTPN6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-221

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRVdGL-4Cre

    Plasmid

    #98039

    Purpose

    Second-generation rabies viral vector genome

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    mCre
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Cis1-SH2

    Plasmid

    #46417

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Cis1 (SOCS1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 90-214

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Socs3-SH2

    Plasmid

    #46506

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs3 (SOCS3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 22-142

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Socs1-SH2

    Plasmid

    #46504

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs1 (SOCS1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-166

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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