We narrowed to 29,615 results for: PLE

1. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

   Plasmid

   #85453

   Purpose

   Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-sgRNA-Backbone-UP-TdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   deletion of AA123-365 in tdTomato (please see dep…

   Promoter

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Plxnb2(S)-Fc-His

   Plasmid

   #72129

   Purpose

   Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb2 (Plxnb2 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAPTrap-mCherry-IRESMygro

   Plasmid

   #82505

   Purpose

   Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   mCherry-IRESMygro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-UBC-S3-ExRaiAKAR

   Plasmid

   #160724

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S3-ExRaiAKAR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-UBC-S4-ExRaiCKAR

   Plasmid

   #160725

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S4-ExRaiCKAR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAPTrap-eGFP-IRESMygro

   Plasmid

   #82503

   Purpose

   Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   eGFP-IRESMygro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pATP416-­ptetO7-­pphlO6-­crtYBI

   Plasmid

   #165976

   Purpose

   Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pphlO6), Synthetic promoter (…

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSNAP-CAPZ

   Plasmid

   #69948

   Purpose

   Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

   Depositor

   Jeff Gelles

   Article

   Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

   Inserts

   CapZ beta 1 (CAPZB Chicken)
   CapZ alpha 1 (CAPZA1 Chicken)
   

   Use

   Tags

   His 6 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBUN501

   Plasmid

   #50582

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   zCas9D10A
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Cas9D10A nickase, was derived from the zCas9 and …

   Promoter

   AtU6-26p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL40C_PGKintron_Cas9_Green

    Plasmid

    #134966

    Purpose

    Lentiviral vector coding Cas9 and mNeonGreen

    Depositor

    Beat Bornhauser

    Article

    Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

    Insert

    Cas9-P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK promoter with beta-globin intron

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTEE

    Plasmid

    #159748

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIP mScarlet-MOSPD2 W201E

    Plasmid

    #186477

    Purpose

    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

    Plasmid

    #111387

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-EGFP-C1-HsNot1iso1_1093-1317_W

    Plasmid

    #147892

    Purpose

    Mammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot1iso1_1093-1317 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid

    #135015

    Purpose

    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    

    Use

    Cryptosporidium expression

    Tags

    mCherry and Nluc-neo

    Expression

    Mutation

    Promoter

    Cryptosporidium actin and enolase

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1097

    Plasmid

    #29028

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid

    #165975

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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