We narrowed to 19,222 results for: Tec

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pAAV-CAG-miniGFP1-P2A-FusionRed

Plasmid

#188732

Purpose

Express miniGFP1 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT3-Neo-EF1a-GFP

Plasmid

#69134

Purpose

Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Type

Empty backbone

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Jan. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1749

Plasmid

#127983

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABEmax

Plasmid

#119815

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl-ENT N-1F (R1103A)

Plasmid

#216548

Purpose

For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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Marathon-RT pentamutant for Split-PE (pEH107)

Plasmid

#190110

Purpose

Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Mutation

D14R, N26R, D74R, N116K, N197R

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCC2)

Plasmid

#235072

Purpose

Expresses Tad-CBE(CCC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-Tad-CBE(ACC2)

Plasmid

#235081

Purpose

Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBJ7

Plasmid

#167136

Purpose

A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Kanamycin promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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