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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,273 results for: cas9

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Showing: 4661 - 4680 of 14273 results

  7. pJM143

    Plasmid
    #134166
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…
    Available Since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134166-plasmid-map-sequence-id-271475

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  9. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  11. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid
    #102888
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102888-plasmid-map-sequence-id-262466

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  13. lenti-EF1a-dCas9-VPR-eGFP

    Plasmid
    #241307
    Purpose
    3rd generation lenti vector encoding dCas9 (s. Pyogenes) fused with the VP64-p65-Rta(VPR) and a 2A GFP fluorescence marker
    Depositor
    Robby Bowles
    Insert
    eGFP
    Use
    Lentiviral
    Available Since
    Nov. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241307-plasmid-map-sequence-id-483039

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  17. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129028-plasmid-map-sequence-id-256131

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  19. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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Showing: 4661 - 4680 of 14273 results