We narrowed to 32,724 results for: eng

1. pORF1-2

   Plasmid

   #206024

   Purpose

   An expression vector for mouse LINE1 ORF1-2

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   ORF1-2 derived from mouse LINE1
   

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA_GFP-T2

   Plasmid

   #41820

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelper_AraC/pBad-Tnpase (camR)

   Plasmid

   #213909

   Purpose

   Arabinose-inducible himar1C9 transposase expression - cam resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   AraC/pBad ATc-inducible himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQAT07

   Plasmid

   #223379

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBES1-LacOx50-blast

   Plasmid

   #210221

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZY101

   Plasmid

   #73932

   Purpose

   This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

   Depositor

   Zhanyuan Zhang

   Article

   Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

   Insert

   bar
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRGEB33

   Plasmid

   #158151

   Purpose

   Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-jGCaMP7b-XN

    Plasmid

    #178362

    Purpose

    Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XN
    

    Expression

    Mammalian

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK_Sox10_enh_10E2_Cherry

    Plasmid

    #130590

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox10_enh_10E2
    

    Use

    Enhancer reporter

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK_Pax7_enh_195_Citrine

    Plasmid

    #130604

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Pax7_enh_195
    

    Use

    Enhancer reporter

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-GfaABC1D-GRAB_Ado1.0mut

    Plasmid

    #153290

    Purpose

    Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytes

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBR5pemIK

    Plasmid

    #126554

    Purpose

    Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection

    Depositor

    Lindsey Burbank, Drake Stenger

    Article

    Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-LIC-V5

    Plasmid

    #120248

    Purpose

    For LIC cloning with C-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ230-D156R (ttLbCas12a)

    Plasmid

    #195366

    Purpose

    A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    LbCas12a-D156R (ttLbCas12a)
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

    Expression

    Plant

    Promoter

    ZmUBI1

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZ148-BhCas12b-sgRNA-scaffold

    Plasmid

    #122448

    Purpose

    Expresses sgRNA for BhCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BhCas12b-sgRNA-scaffold, CMV-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. px458_Conc2

    Plasmid

    #134449

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-PIK3R1

    Plasmid

    #174192

    Purpose

    Expresses PIK3R1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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