We narrowed to 5,840 results for: pcas

1. hPRL1_1-169

   Plasmid

   #209792

   Purpose

   Bacterial expression of human PRL-1 without farnesylation motif

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

   Insert

   PRL-1 (PTP4A1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 1-169 only

   Promoter

   T7

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hPRL1_1-169_C104D

   Plasmid

   #209793

   Purpose

   Bacterial expression of human PRL-1 C104D mutant without farnesylation motif

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

   Insert

   PRL-1 (PTP4A1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 1-169 only; C101D

   Promoter

   T7

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Modular BE4max for GG insertion

   Plasmid

   #198891

   Purpose

   Easily insert deaminase of choice into BE4max using Golden Gate cloning

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL23_Cas9-His Δcys E945C

   Plasmid

   #206284

   Purpose

   Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E945C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL24_Cas9-His Δcys E1026C

   Plasmid

   #206285

   Purpose

   Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1026C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-Cas9-sgHPRT1

   Plasmid

   #196713

   Purpose

   CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Insert

   Cas9-T2A-BSD-U6-sgHPRT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Promoter

   EF1a/hU6

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1300_pUB10_pcoCASphi_E9t_MCS_version1

   Plasmid

   #197937

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. UAS-GFP::NMN-Deamidase

    Plasmid

    #187868

    Purpose

    Gal4/UAS expression of GFP::NMN-Deamidase

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    NMN-Deamidase
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-RARa-VP16

    Plasmid

    #185561

    Purpose

    Doxycycline inducible expression of constitutively active Retinoic Acid Receptor alpha

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    RARa-VP16 (Rara Mouse)
    

    Tags

    VP16

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458_sgRNA_Ago1_5

    Plasmid

    #172470

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458_sgRNA_Ago1_6

    Plasmid

    #172471

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1.

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK617

    Plasmid

    #179297

    Purpose

    Expresses dCas9 fused to VP64

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VP64
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_iRFP713

    Plasmid

    #167860

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-TEVcs- FIP200 delta641-779-MBP

    Plasmid

    #171411

    Purpose

    For the purifcation of human ULK1 truncated complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    FIP200 (RB1CC1 Synthetic)
    

    Expression

    Mammalian

    Mutation

    truncation (delta641-779-MBP)

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TAC-Cas9

    Plasmid

    #120352

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

    Plasmid

    #139963

    Purpose

    Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_058

    Plasmid

    #123984

    Purpose

    Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sg Safetargeting Chr11
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK234_038

    Plasmid

    #123917

    Purpose

    Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA-pSV40
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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