We narrowed to 17,914 results for: REV

1. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. phage UbiC NLS HA stdMCP Halo

   Plasmid

   #104098

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdMCP-Halo
   

   Use

   Lentiviral

   Tags

   C-terminal Halo-tag, N-terminal NLS, and Two syno…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. phage UbiC NLS HA stdPCP stdGFP

   Plasmid

   #104099

   Purpose

   Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdPCP-stdGFP
   

   Use

   Lentiviral

   Tags

   N-terminal NLS, Two synonymous copies of GFP (C-t…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Braf

   Plasmid

   #40775

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   Braf (BRAF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Starting Met removed

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VAMP8-mCherry

   Plasmid

   #92424

   Purpose

   Expression of VAMP8 C-terminally conjugated to mCherry.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP8 (Vamp8 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   A36S (Please see Depositor Comments below)

   Promoter

   CMV

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSN067

   Plasmid

   #101748

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. alpha catenin conformation sensor

   Plasmid

   #71709

   Purpose

   This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin FRET Sensor
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMV361_MjTyrRS

    Plasmid

    #193250

    Purpose

    Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

    Depositor

    Jessica Seeliger, Peter Schultz

    Article

    Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

    Insert

    MjTyrRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hsp60

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge