We narrowed to 14,212 results for: cas9 genes

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pMEL11

Plasmid

#107917

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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PEA1-GFP

Plasmid

#171993

Purpose

Delivers all prime editing nickase components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PEA1-Puro

Plasmid

#171991

Purpose

Delivers all prime editing nickase components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_T7-PE2-Nuclease

Plasmid

#171997

Purpose

Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CMV_T7-Cas9-RT

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

Plasmid

#169921

Purpose

DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

IRES-Puro-pEF1a-EGFP

Use

CRISPR

Promoter

EF1a

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBFv-U6.2

Plasmid

#138400

Purpose

gRNA expression vector for Drosophila

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Type

Empty backbone

Expression

Insect

Promoter

U6-2

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pQCH

Plasmid

#164160

Purpose

IncQ BHR plasmid

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS257

Plasmid

#73716

Purpose

SapTrap destination vector for building repair template only vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SapI repair template insertion site

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS272

Plasmid

#73728

Purpose

SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded C-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS281

Plasmid

#73732

Purpose

SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded N-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS285

Plasmid

#73734

Purpose

SapTrap egl-13 NLS connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

egl-13 NLS

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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AByH

Plasmid

#164152

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByD

Plasmid

#164150

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByC

Plasmid

#164149

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByB

Plasmid

#164148

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByA

Plasmid

#163749

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByI

Plasmid

#164153

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Availability

Academic Institutions and Nonprofits only

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