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pD649-HAsp-CEACAM8-COMP5AP-AviTag-9xHis

Plasmid

#157294

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM8 (CEACAM8 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NFAM1-COMP5AP-AviTag-9xHis

Plasmid

#157066

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NFAM1 (NFAM1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R363L

Plasmid

#180381

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R363L (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R363L

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_D35N_R49Q

Plasmid

#180383

Purpose

lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_D35N/R49Q (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

D35N/R49Q

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R49H

Plasmid

#180382

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R49H (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R49H

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-10R

Plasmid

#178664

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-10R (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

ten Arg residues removed

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+8D

Plasmid

#178674

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+8D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

eight Asp residues added

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+7R

Plasmid

#178667

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+7R (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

seven Arg residues added

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-2K

Plasmid

#178668

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with two Lys residues removed (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-2K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

two Lys residues removed

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+4D

Plasmid

#178673

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+4D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

four Asp residues added

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-6R

Plasmid

#178665

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues removed (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-6R (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

six Arg residues removed

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+2R

Plasmid

#178666

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+2R (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

two Arg residues added

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD-6R+6K

Plasmid

#178670

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-6R+6K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

six Arg residues replaced with Lys

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+12D

Plasmid

#178675

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Asp residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+12D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twelve Asp residues added

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-3R+3K

Plasmid

#178669

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-3R+3K (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

three Arg residues replaced with Lys

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD+7F-7Y

Plasmid

#178660

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD +7F-7Y (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

seven Tyr residues replaced with Phe

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+12E

Plasmid

#178676

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+12E (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twelve Glu residues added

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pME-MmCav1

Plasmid

#170527

Purpose

Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Multisite gateway entry clone

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAPL318E

Plasmid

#166444

Purpose

Bacterial expression of 6x His tagged YAPL318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAPL318E (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP4LE

Plasmid

#166445

Purpose

Bacterial expression of 6x His tagged YAP4LE

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP4LE (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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