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pAC-EHER

Plasmid

#53262

Purpose

Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

crtE, idi, crtX, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Publication

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX330-NEAT1pr_v1

Plasmid

#97081

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Publication

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v1

Use

CRISPR

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6/sgClta-sfGFP-Clta

Plasmid

#200261

Purpose

U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

Depositor

Justin Eyquem

Publication

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta

Use

AAV

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F1bbs kan GCGTGGNNN

Plasmid

#18753

Depositor

Scot Wolfe

Publication

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F3bbs kan NNNTGGGCGk

Plasmid

#18756

Depositor

Scot Wolfe

Publication

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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hMLKL NB(1-182)-2xFv-Venus

Plasmid

#106077

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

Plasmid

#31480

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (ani target)

Use

Lentiviral

Mutation

mCherry has M9S, M16L to reduce background mCherr…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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p426 25Q GAL

Plasmid

#1187

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Publication

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD47-T2A-GFP

Plasmid

#205449

Purpose

lentiviral plasmid for expression of mouse CD47

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD47 (Cd47 Mouse)

Expression

Mammalian

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMag(3x)-iRFP

Plasmid

#67305

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMag(3x)-iRFP (Tiam1 Mouse, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

Plasmid

#135455

Purpose

Bacterial expression of uTEV1Δ protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability, S153N mutation…

Promoter

tac promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-hLIF

Plasmid

#179711

Purpose

Expresses H6-MBP-LIF

Depositor

Mark Arbing

Insert

Leukemia inhibitory factor (LIF Human)

Tags

His6-MBP-TEV protease site

Expression

Bacterial

Promoter

tac

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-2

Plasmid

#161819

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Publication

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAR17_StrepI_hFASh_H8_pET22b

Plasmid

#122846

Purpose

Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Publication

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

human FASN/ human fatty acid synthase (FASN Human)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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