We narrowed to 6,096 results for: pcDNA 3.1

1. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45216

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   Histone H2B-targeted Aurora B FRET sensor
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOVC1_TtCarH-CBD_X

   Plasmid

   #131179

   Purpose

   Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   TtCarH-CBD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOVC1_MxCarH-CBD_X

   Plasmid

   #131178

   Purpose

   Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   MxCarH-CBD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH607-Tier1-PCREm4-Nluc

   Plasmid

   #169594

   Purpose

   Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCerulean-hCOL1A1

   Plasmid

   #140112

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mito-LSSmGFP

   Plasmid

   #186536

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-ShadowY-kCAAX

   Plasmid

   #115564

   Purpose

   dark ITRACK acceptor construct for dual color imaging

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   ShadowY with KRas membrane targeting domain
   

   Tags

   ShadowY

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. tCRMod-GGSGGS9

   Plasmid

   #111762

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS9-mRuby2 fusion
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH231-Tier1-PmPGK1-mTagBFP2

   Plasmid

   #169520

   Purpose

   Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cenp-B DBD INCENP GFP

    Plasmid

    #45237

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD, INCENP-Δcen, GFP
    

    Tags

    DNA binding domain of CENP-B and GFP

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFLAG-ZYG11A

    Plasmid

    #110551

    Purpose

    expresses a FLAG-tagged human ZYG11A protein in mamalian cells

    Depositor

    Edward Kipreos

    Article

    Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

    Insert

    ZYG11A (ZYG11A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. ER2-mGreenLantern

    Plasmid

    #186548

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    mGreenLantern
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ER2-LSSmGFP

    Plasmid

    #186538

    Purpose

    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2386-Tier1-PhCMV-GCaMP6s

    Plasmid

    #169519

    Purpose

    Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER2-hfYFP

    Plasmid

    #186518

    Purpose

    Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fra-1

    Plasmid

    #187906

    Purpose

    Expresses Fra-1 in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    Fra1 (Fosl1 Rat)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. optGluCl alpha EYFP

    Plasmid

    #15104

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl alpha (glc-1 Nematode)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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