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pcDNA4TO-5xGCN4_v4-kif18b-24xPP7

Plasmid

#74927

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

5xGCN4_v4-kif18b-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Article

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74928

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b

Plasmid

#74934

Purpose

Translation reporter that lacks PP7 sites in the 3'UTR

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pJF076Sa

Plasmid

#113322

Purpose

J1-mRFP1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPro24(s)-gfp

Plasmid

#17813

Depositor

Jay Keasling

Article

Lee et al Biotechnol Bioeng. 2006 Apr 5. 93(5):912

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

D234N compared to AF007834

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pRHA12

Plasmid

#138942

Purpose

AND gate with IPTG and aTc as inputs and sfGFP as output

Depositor

Guillermo Rodrigo

Article

Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

Insert

riboregulated sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP_Z_Cav1_H6_Periplasmic_mScarlet

Plasmid

#223703

Purpose

To produce caveospheres with periplasmically encapsulated mScarlet

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP_Z_Cav1_H6_Periplasmic_mScarlet

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRHA40

Plasmid

#138941

Purpose

XOR gate with IPTG and aTc as inputs and mRFP1 as output

Depositor

Guillermo Rodrigo

Article

Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

Insert

riboregulated mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1228

Plasmid

#11410

Depositor

Sung-Hou Kim

Article

Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

Insert

eukaryotic translation initiation factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLPT107

Plasmid

#85525

Purpose

Repressilator with triple reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI
mKate2

Use

Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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p663-UBC-PM-TurboID-V5_IDG-K

Plasmid

#214455

Purpose

Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PM-TurboID-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT119

Plasmid

#85526

Purpose

Repressilator without degradation tags, with integrated reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEPkan-S2

Plasmid

#61601

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Osr1

Plasmid

#26485

Depositor

Paul Danielian

Article

So et al Mech Dev. 1999 Jun . 84(1-2):157-60.

Insert

Osr1 (Osr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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p663-UBC-ER-TurboID-V5_IDG-K

Plasmid

#214453

Purpose

Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

ER-TurboID-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAID5.1-N

Plasmid

#145808

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFIP

Plasmid

#17565

Depositor

Jay Keasling

Article

Ham et al Biotechnol Bioeng. 2006 May 5. 94(1):1-4

Insert

inversion switch

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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