We narrowed to 33,901 results for: CMV

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5'-3xFLAG GFP minimal CMV pCDNA5

Plasmid

#212086

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (L115R)- HaloTag

Plasmid

#205712

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

HaloTag and XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DEAD XPACK-DNAJC5 (L115R)

Plasmid

#205718

Purpose

Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

Dead XPACK (GCINIKRKD)

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔC10 (Δ189-198)

Plasmid

#205720

Purpose

Mammalian expression of DNAJC5-ΔC10 (Δ189-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

Δ189-198

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔC20 (Δ179-198)

Plasmid

#205721

Purpose

Mammalian expression of DNAJC5-ΔC20 (Δ179-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

Δ179-198

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔC40 (Δ151-198)

Plasmid

#205723

Purpose

Mammalian expression of DNAJC5-ΔC40 (Δ151-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

Δ151-198

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-CMV(trunc)-Parkin-A92mKO2

Plasmid

#213549

Purpose

Lentiviral vector for stable low expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Truncated CMV

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

Plasmid

#209199

Purpose

Mutagenesis of Kcnma1

Depositor

Larry Zweifel

Article

Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

Insert

Kcnma1 (Kcnma1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 321-843 REV1

Plasmid

#201587

Purpose

Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 321-843 of DNA repair prote…

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

Plasmid

#201466

Purpose

Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 111-136

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

Plasmid

#201467

Purpose

Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 137-160

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

Plasmid

#201468

Purpose

Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 202-230

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

Plasmid

#201469

Purpose

Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 287-316

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-L2D/V9D/I19D PPM1H

Plasmid

#207341

Purpose

Mammalian expression of HA-L2D/V9D/I19D PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

L2D/V9D/I19D

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-MBP-rATOM(WDR5)

Plasmid

#209710

Purpose

Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NLS-MBP-rATOM(WDR5)

Tags

NLS-MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Flag-LRRK2 K17A/K18A

Plasmid

#186012

Purpose

Flag-LRRK2 K17A/K18A

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

Flag-LRRK2 K17A/K18A (LRRK2 Human)

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-miniSdd3-BE4max-BlastR

Plasmid

#204849

Purpose

For cytosine base editing using miniSdd3 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BRAF-REG-T241P-Halo

Plasmid

#202549

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Threonine 241 mutated to proline

Promoter

CMV

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

Plasmid

#194016

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.2)
eGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

cmb and u6

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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