We narrowed to 19,017 results for: Tec;

1. ABE-RA4.2

   Plasmid

   #208307

   Purpose

   Expresses ABE-RA4.2 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA4.2-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

   Plasmid

   #191144

   Purpose

   Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1:EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBY3_ligD-HygR

   Plasmid

   #104625

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   FnCpf1 codon-optimized C.glutamicum

   Promoter

   hsp60

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. UBQ10:sXVE:S10-(MCS)

   Plasmid

   #108177

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCALNL-tdTomato

   Plasmid

   #125573

   Purpose

   Cre-dependent expression of tdTomato in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   tdTomato
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuroATT

   Plasmid

   #47539

   Purpose

   Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

   Depositor

   David Weinstock

   Article

   Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pRDA_112

   Plasmid

   #136475

   Purpose

   Lentiviral expression of AsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   AsCas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM113

   Plasmid

   #127210

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   WUS2, AtSTM, sgRNA, Luc
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-nCas9-PBE

   Plasmid

   #98160

   Purpose

   Expresses CRISPR base editor in maize

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Maize

   Expression

   Plant

   Promoter

   Ubi

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EasyFusion Halo-mAID

    Plasmid

    #112852

    Purpose

    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Halo-mAID
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CL7-LbCas12a

    Plasmid

    #164658

    Purpose

    Bacterial expression of LbCas12a using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a
    

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104841

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    b2AR-eLOV-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNubWtXgate

    Plasmid

    #105079

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Tags

    NubI

    Expression

    Yeast

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9 Null

    Plasmid

    #198727

    Purpose

    Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-LOV-VP16

    Plasmid

    #42499

    Purpose

    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

    Depositor

    Ricardo Dolmetsch

    Article

    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

    Insert

    FKF1 (N-LOV, G128D mutant) fused with VP16
    

    Tags

    F-type VP16 activation domain and HA tag

    Expression

    Mammalian

    Mutation

    G128D 1-168aa

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRB_SCF2_LD6_CTEV

    Plasmid

    #58879

    Purpose

    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. UPB-duodenum (UPB6)

    Plasmid

    #180785

    Purpose

    Unique Projection Barcode (UPB6)-Duodenum for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM4D(Gi) (partial )
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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