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We narrowed to 8,596 results for: dykddddk

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Showing: 4741 - 4760 of 8596 results

  4. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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  5. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

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  6. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  7. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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  8. pCAG-hPPP3CC-FLAG

    Plasmid
    #179162
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179162-plasmid-map-sequence-id-352821

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  9. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid
    #137723
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Deleted amino acids 600-682
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137723-plasmid-map-sequence-id-288120

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  10. pTF-ACE2s

    Plasmid
    #162786
    Purpose
    The extracellular domain of Angiotensin converting enzyme 2 (ACE2) expression
    Depositor
    Ivan Vorobiev
    Article
    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    10xHis, FLAG, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162786-plasmid-map-sequence-id-333795

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  15. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  16. MmSphk2

    Plasmid
    #118598
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available Since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    118598-plasmid-map-sequence-id-236037

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  17. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid
    #170228
    Purpose
    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    Fusion
    Promoter
    EF1a
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170228-plasmid-map-sequence-id-334409

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  18. LV-AR-AAFF

    Plasmid
    #171218
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-AAFF (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-K580A-V581A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171218-plasmid-map-sequence-id-342447

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  20. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

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Showing: 4741 - 4760 of 8596 results