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pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127325

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

Plasmid

#127320

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

Plasmid

#127313

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

Plasmid

#127319

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127324

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG

Plasmid

#127311

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG

Plasmid

#127310

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG

Plasmid

#127329

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG

Plasmid

#127328

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127327

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127326

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

Plasmid

#61796

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

Plasmid

#61793

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

Plasmid

#61791

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

Plasmid

#61790

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

Plasmid

#61786

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
shCTL-mlpx-neo

Plasmid

#65233

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

Plasmid

#61787

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR with miR-200c MRE-2 mutated

Use

Luciferase

Mutation

miR-200c MRE-2 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

Plasmid

#61788

Purpose

Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Tob1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only