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p414TEF_Hsp82_noTER

Plasmid

#61714

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

TEF1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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p414CYC_Hsp82_noTER

Plasmid

#61716

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

CYC1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV/cmGAD67 GFP WPRE SV40pA

Plasmid

#245935

Purpose

GABAergic neuron-specific cmGAD67 promoter

Depositor

Hirokazu Hirai

Publication

Fukai et al Mol Ther. 2026 Jun 25:S1525-0016(26)0048

Insert

Mouse GAD67 promoter, Region ii to Etss (Transcriptional Start Site in Exon1)

Use

AAV

Tags

GFP

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCGT002_nuclear-EGFP

Plasmid

#248084

Purpose

Constitutive expression of NLS-tagged EGFP from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Publication

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

EGFP

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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3xFLAG-Halo-Dyskerin

Plasmid

#207578

Purpose

CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

HaloTag DKC1

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hRIP3 dRHIM

Plasmid

#208788

Purpose

Inducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

RIP3 (RIPK3 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

RHIM domain is removed

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ePPE-V223A

Plasmid

#205243

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

SV40_NLS-nCas9-NC-MLV-SV40_NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-RPE65

Plasmid

#206346

Purpose

AAV plasmid expressing RPE65 in photoreceptors

Depositor

Connie Cepko

Publication

Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

Insert

Rpe65 (Rpe65 Mouse)

Use

AAV

Mutation

450-Met (C57BL6 natural variation)

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdKatushka2-WPRE

Plasmid

#193337

Purpose

Expresses tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Publication

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Expression

Mammalian

Promoter

TRE

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3755 : HDR-HsIRE1a-10xGS-HaloTag

Plasmid

#185677

Purpose

HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.

Depositor

Peter Walter

Publication

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 homology arms (ERN1 Synthetic, Human)

Use

CRISPR

Tags

HaloTag

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA D463N

Plasmid

#195203

Purpose

Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

Depositor

David Bryant, Peter Parker

Publication

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Mutation

D463N

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pElectra2-LaminA

Plasmid

#179483

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2-LaminA

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPaxillin-Electra2

Plasmid

#184926

Purpose

Expresses paxillin fused to blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

paxillin-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_miniABEmaxNG(GG)

Plasmid

#187062

Purpose

miniABEmax(GG)-SpCas9-NG

Depositor

Renzhi Han

Publication

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax(GG)-SpCas9-NG

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Publication

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGECInano

Plasmid

#186188

Purpose

AAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicator

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Use

AAV

Promoter

CAG

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Expression

Bacterial

Mutation

Wild-type

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Publication

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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