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mGarnet-RITA

Plasmid

#104313

Purpose

codes for a fusion of fluorescent protein mGarnet and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

mGarnet-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFP1F5

Plasmid

#165064

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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LEXY TR (with KRAB domain) (pDN163)

Plasmid

#72662

Purpose

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GGE252

Plasmid

#120791

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown_GSY-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GGE253

Plasmid

#120790

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsUp_GSY-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-probe1

Plasmid

#105454

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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p8203 MSCV-neo C-V5 16E7

Plasmid

#193976

Purpose

HPV16 E7 expression, C-terminal V5 tag

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

HPV16 E7

Use

Retroviral

Tags

V5

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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reporter-gT2

Plasmid

#47321

Purpose

TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
OMP-SpH-ACNF TV

Plasmid

#15513

Depositor

Peter Mombaerts

Article

Bozza et al Neuron. 2004 Apr 8. 42(1):9-21.

Insert

olfactory marker protein (Omp Mouse)

Use

Mouse Targeting

Mutation

OMP coding region and 3' end were replaced b…

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
p817-mgammaFcry-mCherry

Plasmid

#23154

Depositor

Zbynek Kozmik

Article

Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Insert

mouse gammaF crystallin promoter:mCherry (Crygf Mouse)

Use

Multicloning site flanked by i-scei sites

Tags

mCherry

Mutation

mouse gammaF crystallin promoter (-225 - +73) pre…

Available Since

April 13, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-hphMX4

Plasmid

#26883

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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S13_EcoRV_UBC_AscI

Plasmid

#128477

Purpose

UBC promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

UBC promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-zDrosha

Plasmid

#171540

Purpose

Encodes Zebrafish Drosha protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

drosha (rnasen Zebrafish)

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-NT

Plasmid

#17594

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-NT (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 49-498

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11197

Plasmid

#191784

Purpose

SpCas9 expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas9 +13 introns-T-E9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry-RT-mCherry

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pHPdestVenus

Plasmid

#24568

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

Venus

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pISH-Cck

Plasmid

#105989

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

cholecystokinin (Cck Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc

Plasmid

#24559

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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