We narrowed to 5,339 results for: pcdna3.1

1. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. S1 1029 AIDx with UGI

   Plasmid

   #135354

   Purpose

   Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   hAIDx-nSpyCas9 aa-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. S1 1029 CP ABEMax strategy 2

   Plasmid

   #135358

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   WT Tada-nSpyCas9 aa 1029-evolved Tada
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. S1 1029 CP ABEMax strategy 3

   Plasmid

   #135359

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 CP nSpyCas9 ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. S1 1029 miniABEMax V82G strategy1

   Plasmid

   #135360

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   mini ABEMax 1029 nSpyCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. S1 1029 V82G ABEMax Strategy 3

   Plasmid

   #135363

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 V82G ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAL4-DBD-HOXD11wt-IDR

   Plasmid

   #145242

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD11wt-IDR (HOXD11 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGAL4-DBD-HOXD11-Adel-IDR

   Plasmid

   #145241

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD11-Adel-IDR (HOXD11 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAL4-DBD-HOXA13+7A-IDR

   Plasmid

   #145239

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13+7A-IDR (HOXA13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAL4-DBD-HOXA13-44A-IDR

    Plasmid

    #145237

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-44A-IDR (HOXA13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAL4-DBD-HOXA13-15A-IDR

    Plasmid

    #145236

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-15A-IDR (HOXA13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGAL4-DBD-HEY2wt-IDR

    Plasmid

    #145235

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HEY2wt-IDR (HEY2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAL4-DBD-GSX2wt-IDR

    Plasmid

    #145233

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2wt-IDR (GSX2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGAL4-DBD-EOMES-Adel-IDR

    Plasmid

    #145230

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    EOMES-Adel-IDR (EOMES Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAL4-DBD-HOXD13wt-IDR

    Plasmid

    #145244

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXA13 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAL4-DBD-TBX1-Adel-IDR

    Plasmid

    #145253

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and TBX1-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBX1-Adel-IDR (TBX1 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAL4-DBD-GSX2-Adel-IDR

    Plasmid

    #145232

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2-Adel-IDR (GSX2 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPK-352

    Plasmid

    #157922

    Purpose

    pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    tPcyA
    IRES
    HO1
    FD
    FNR
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    Mitochondrial Targeting Sequence, Mitochondrial T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Mm_Plk5(300-595) PBD domain

    Plasmid

    #136316

    Purpose

    Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Expression

    Mammalian

    Mutation

    fragment 300-595

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-Mm_Plk5(D168A)

    Plasmid

    #136318

    Purpose

    Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Expression

    Mammalian

    Mutation

    D168A

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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