179,918 results
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Plasmid#174051PurposeGateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assayDepositorTypeEmpty backboneUseLuciferaseExpressionPlantAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pLenti-PE2-BSD
Plasmid#161514PurposeExpression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selectionDepositorInsertPE2-P2A-BSD
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEF-1α core promoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV Retrograde)
Viral prep#154868-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV2)
Viral prep#105537-AAV2PurposeReady-to-use AAV2 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-CXCR4
Plasmid#98942PurposeMammalian expression plasmid for untagged human CXCR4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR4 (CXCR4 Human)
ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDG458
Plasmid#100900PurposeSpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAGExpressionMammalianPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)
Plasmid#103864PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianPromoterCAGAvailable sinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTH100
Plasmid#84458PurposepJB38-NWMN29-30 + SarA_P1-sGFP-TermDepositorInsertSarA_P1-sGFP from pCM29
Tags6xHis (on sfGFP)Available sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-hSynapsin-Cre-WPRE
Plasmid#86641PurposeExpresses Cre in mammalian cellsDepositorInsertCre
UseCre/Lox, Lentiviral, Mouse Targeting, and Synthet…ExpressionMammalianPromoterhSynapsinAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLS-mP
Plasmid#81225PurposeLentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.DepositorTypeEmpty backboneUseLentiviralPromoterDerived from pGL4.23Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralExpressionMammalianPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2C-3XtbGFP-PEST
Plasmid#69072PurposeExpresses 3XturboGFP-NLS-PEST under MERVL_LTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertturboGFP
ExpressionMammalianAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNase HI
TagsNLS from SV40 T antigen and mCherryExpressionMammalianPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Vinculin-23
Plasmid#55159PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (VCL Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by